Certificate

Brand

Product Type

Instrument

Highlights

Label or Dye 6-FAM, LIZ, NED, SID, TAZ, VIC Product Line GlobalFiler™ Product Type PCR Amplification Kit Shipping Condition Dry Ice Target Loci Amelogenin, CSF1PO, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D1S1656, D21S11, D22S1045, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, DYS391, FGA, IQC-Large, IQC-Small, SE33, TH01, TPOX, Y indel, vWA

Application

Features of the GlobalFiler IQC PCR Amplification Kit include:
• Internal quality control (IQC) system enables detection of and discrimination between amplification inhibition and DNA sample degradation and an in-sample positive amplification control, providing actionable information
• Efficient protocols minimize amplification time compared to previous-generation kits
• An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
• Enhanced intracolor balance simplifies interpretation of mixture samples
• The inclusion of 10 mini-STRs maximizes results from degraded samples (<220 bp)
• Contains all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility
• Improved data interpretation with reduced pull-up edits and off-scale data recovery when combined with 3500 Data Collection Software v4.0 and GeneMapper ID-X Software 1.6
• Manufactured to IS0 18385 standards

Specifications

Label or Dye 6-FAM, LIZ, NED, SID, TAZ, VIC
Product Line GlobalFiler™
Product Type PCR Amplification Kit
Shipping Condition Dry Ice
Target Loci Amelogenin, CSF1PO, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D1S1656, D21S11, D22S1045, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, DYS391, FGA, IQC-Large, IQC-Small, SE33, TH01, TPOX, Y indel, vWA

Other information

The GlobalFiler IQC kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages six dyes for the incorporation of additional markers, generating a highly discriminating kit for use by the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, to help deliver optimized results with even highly degraded samples.

Developed specifically for casework applications, the GlobalFiler kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The addition of the IQC provides more insight into sample quality to assist in distinguishing between inhibition and degradation while also providing a positive control for amplification. The GlobalFiler IQC kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM Select and Identifiler primer sequences helps ensure maximum concordance with previously typed samples. In addition, the GlobalFiler IQC kit contains multiple gender markers to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience matters
Thermo Fisher Scientific designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

Contents & Storage

Store at -15° to -25°C upon receipt, and 2° to 8°C after initial use for up to 6 months.

Related Products

SeqStudio™ 8 Flex Genetic Analyzer

The Applied Biosystems SeqStudio 8 Flex Genetic Analyzer offers design improvements and technological advances to the capillary electrophoresis (CE) workflow. It delivers gold-standard Sanger sequencing and fragment analysis data quality with increased flexibility, easier operation, and cloud connectivity to enable researchers to manage their workloads and work/life balance effectively. Designed for ultimate ease-of-use, this mid-throughput system offers high quality, excellent reliability, and an optimal user experience for researchers who want to work smarter in the lab.

3500xL Genetic Analyzer

The 3500xL Genetic Analyzer is a 24-capillary sequencing platform that can be used for a wide variety of applications, including de novo sequencing and resequencing (mutational profiling), as well as microsatellite analysis, MLPA™, LOH, MLST, and SNP validation or screening. The majority of applications can be run on a single polymer and capillary array.

QuantStudio™ Absolute Q™ Digital PCR System, desktop

The Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR System combines the power of absolute quantification via digital PCR with the simplicity of a qPCR workflow. With a single hands-on step that takes 5 minutes to complete, digital PCR is now easier than ever. The QuantStudio Absolute Q system provides a high-quality, consistent, and easy-to-use solution for researchers who want to join the quantification revolution.

QuantStudio™ 7 Pro Real-Time PCR System, TaqMan™ Array Card, desktop

The Applied Biosystems QuantStudio 7 Pro Real-Time PCR System adds innovative smart features to the real-time PCR (qPCR) workflow, all in a compact footprint. With facial authentication, voice commands, Smart Help, multiplex capability, motorized interchangeable blocks, simplified workflow, intuitive software, automation capabilities, and large capacitive touch-screen interface, the QuantStudio 7 Pro system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Designed for ultimate ease-of-use, the system offers high quality, high throughput, excellent reliability, and an optimal user experience for researchers who want to work smarter in the lab.

QuantStudio™ 7 Pro Real-Time PCR System, TaqMan™ Array Card, desktop

The Applied Biosystems QuantStudio 7 Pro Real-Time PCR System adds innovative smart features to the real-time PCR (qPCR) workflow, all in a compact footprint. With facial authentication, voice commands, Smart Help, multiplex capability, motorized interchangeable blocks, simplified workflow, intuitive software, automation capabilities, and large capacitive touch-screen interface, the QuantStudio 7 Pro system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Designed for ultimate ease-of-use, the system offers high quality, high throughput, excellent reliability, and an optimal user experience for researchers who want to work smarter in the lab.

Infinium KaryoMap v2

The kit contains BeadChips and reagents to perform the KaryoMap v2 assay sufficient for 16 samples.

Disclaimer: This product is labelled for Research Use Only – Not for use in diagnostic procedures.

QuantStudio™ 12K Flex Real-Time PCR System

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray® components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

GlobalFiler™ Express PCR Amplification Kit

The GlobalFiler Express PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler Express kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler PCR Amplification Kit.

ProFlex™ 96-well PCR System

The ProFlex 96-well PCR system is the latest in our line of Applied Biosystems thermal cyclers. The ProFlex system combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.

The ProFlex 96-well PCR system allows you to:
• Interchange the 96-well 0.2-mL block with another block type if throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate an old instrument with Thermal Simulation modes
• Optimize PCR quickly and efficiently with better-than-gradient VeriFlex blocks

HID VeritiPro™ Thermal Cycler, 96 well, 0.2 mL

The HID VeritiPro Thermal Cycler features a 96-well (0.2 mL) format with six independent temperature zones to provide precise temperature control and rapid temperature changes for conducting polymerase chain reactions (PCR). The HID VeritiPro Thermal Cycler features a powerful color touch screen and a USB port to simplify instrument setup and use.