Certificate

Brand

Product Type

Instrument

Highlights

Label or Dye 6-FAM, LIZ, NED, SID, TAZ, VIC Product Line GlobalFiler™ Product Type PCR Amplification Kit Shipping Condition Dry Ice Target Loci Amelogenin, CSF1PO, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D1S1656, D21S11, D22S1045, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, DYS391, FGA, IQC-Large, IQC-Small, SE33, TH01, TPOX, Y indel, vWA

Application

Features of the GlobalFiler IQC PCR Amplification Kit include:
• Internal quality control (IQC) system enables detection of and discrimination between amplification inhibition and DNA sample degradation and an in-sample positive amplification control, providing actionable information
• Efficient protocols minimize amplification time compared to previous-generation kits
• An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
• Enhanced intracolor balance simplifies interpretation of mixture samples
• The inclusion of 10 mini-STRs maximizes results from degraded samples (<220 bp)
• Contains all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility
• Improved data interpretation with reduced pull-up edits and off-scale data recovery when combined with 3500 Data Collection Software v4.0 and GeneMapper ID-X Software 1.6
• Manufactured to IS0 18385 standards

Specifications

Label or Dye 6-FAM, LIZ, NED, SID, TAZ, VIC
Product Line GlobalFiler™
Product Type PCR Amplification Kit
Shipping Condition Dry Ice
Target Loci Amelogenin, CSF1PO, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D1S1656, D21S11, D22S1045, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, DYS391, FGA, IQC-Large, IQC-Small, SE33, TH01, TPOX, Y indel, vWA

Other information

The GlobalFiler IQC kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages six dyes for the incorporation of additional markers, generating a highly discriminating kit for use by the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, to help deliver optimized results with even highly degraded samples.

Developed specifically for casework applications, the GlobalFiler kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The addition of the IQC provides more insight into sample quality to assist in distinguishing between inhibition and degradation while also providing a positive control for amplification. The GlobalFiler IQC kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM Select and Identifiler primer sequences helps ensure maximum concordance with previously typed samples. In addition, the GlobalFiler IQC kit contains multiple gender markers to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience matters
Thermo Fisher Scientific designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

Contents & Storage

Store at -15° to -25°C upon receipt, and 2° to 8°C after initial use for up to 6 months.

Related Products

EmbryoMap

EmbryoMap for labs assessing preimplantation embryo biopsy samples

Through evolution and innovation, the EmbryoMap solution has been analytically validated for detection of whole- and sub-chromosomal imbalances with highly reproducible copy-number, using cell line reference material and embryo biopsy samples

Samples are prepared for sequencing using the EmbryoMap Sample Prep Kit, sequenced on the Illumina MiSeq Sequencing System and data analysis and reporting performed using eMap software

QuantStudio™ 12K Flex Real-Time PCR System

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray® components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

VeriFiler™ Plus PCR Amplification Kit

The Applied Biosystems VeriFiler Plus PCR Amplification Kit is a highly discriminating, highly sensitive, robust kit optimized for forensic casework. Utilizing our 6-dye chemistry, the VeriFiler Plus kit targets 25 markers: 23 autosomal STRs, including Penta D, Penta E, and two gender discrimination markers. These markers meet all of the requirements CODIS markers  as well as the Chinese National autosomal DNA database. The VeriFiler Plus kit was specifically developed to maximize sensitivity to enable maximum information recovery from challenging casework samples including touch, degraded, and inhibited samples, yet can also be run in direct mode for single source reference samples in database workflows. The VeriFiler Plus kit includes all of the same markers as the VeriFiler Express and Huaxia Platinum kits, but in a complementary layout to maximize discrimination for degraded samples. The VeriFiler Plus kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. As with all Applied Biosystems STR kits, the VeriFiler Plus kit is ISO18385 Forensic DNA Grade, giving you piece of mind when using this kit for your casework samples.

HID Nimbus™ Presto System

The Applied Biosystems HID NIMBUS Presto System is an easy-to-use, compact instrument that combines the KingFisher Presto Purification System with an industry leading automated liquid handling workstation to precisely purify nucleic acids from lysed samples for use in sensitive downstream analyses such as DNA quantification and STR amplification.

7500 Real-Time PCR System for Human Identification with Dell Notebook D520

The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.

This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.

EZ DNA Methylation-Gold® Kits

• Consolidated Workflow: DNA denaturation and bisulfite conversion process in one step.
• High Yield: Desulfonation and purification on a spin-column allows elution of bisulfite-converted DNA in as little as 10 µl.
• Suitable for Various Downstream Applications: Bisulfite-converted DNA can be robustly PCR amplified for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

Basic Yellow 40

Product Information

Model: Basic Yellow

Manufacturer: Lynn Peavey

Origin: United States

Rhodamine 6G

Product Information

Model: Rhodamine 6g

Manufacturer: Lynn Peavey

Origin: United States

CytoFLEX

Model: CytoFLEX
Manufacturer: Beckman Coulter Life Sciences/ USA

The CytoFLEX Flow Cytometer is the most innovative flow cytometer system of Beckman Coulter. With compact design and high efficiency in work, CytoFlex provides and easy to use system with the impressive performance you need. Our system allows the researchers just focus on the science with no worry about instrument. Its superior sensitivity and resolution throughout all configurations give it the edge over other cytometry systems four times its size.

– Up to 3 lasers Violet-Blue-Red (V-B-R) and fully activated with 13 colors

– Ability to measure 100 nm particles with violet laser

– Includes 13 band pass filters: 450/45, 525/40 (2), 585/42, 610/20 (2), 660/10 (2), 690/50, 712/25, 780/60 (3)

– For higher throughput applications an optional plate loader module is available

– Smart software in acquisition and analysis CytExpert

TruSight HLA v2 Sequencing Panel

TruSight HLA v2 Sequencing Panel is a HLA library preparation kit for HLA sequencing on Next Generation Sequencer of Illumina.

Human leukocyte antigen (HLA) plays a significant role in the ability of the immune system to recognize and remove invasive, foreign, infected, and malfunctioning cells to fight disease and maintain overall normal health.
HLA mutations can produce aberrant immune response and have been associated with autoimmune disorders, cancer, transplant rejection, and drug sensitivity.