Certificate

Brand

Thermo Fisher Scientific

Product Type

Instrument

Highlights

The 3500xL Genetic Analyzer is a 24-capillary sequencing platform that can be used for a wide variety of applications, including de novo sequencing and resequencing (mutational profiling), as well as microsatellite analysis, MLPA™, LOH, MLST, and SNP validation or screening. The majority of applications can be run on a single polymer and capillary array.

Application
Features of the 3500xL Genetic Analyzer include:
• Single-line, 505 nm, solid-state. long-life laser that uses a standard power supply and requires no heat-removal ducting
• Powerful, integrated data collection and primary analysis software provides real-time assessment of data quality
• Radio frequency identification (RFID) technology tracks key consumables data and records administrative information
• Advanced multiplexing capabilities for DNA fragment analysis with up to six unique dyes
• Unrivaled application flexibility—one array and one polymer are used for most applications
• Simple setup, operation, and maintenance
Specifications

– Number of capillaries: 24 capillaries
– Compatible capillary length: 36 cm or 50 cm
– Number of fluorescent dyes: 6 colors
– Capacity: 2 plates, compatible with 96-well plates, 384-well plates or 8-tube strips
– Compatible with dye sets: E, Z, F, E5, D, G5, J6, J6-T
– Polymer: POP-4™, POP-6™, POP-7™
‘- Sample throughput: ≥1104 samples/day for sequencing, ≥1272 for fragment analysis
‘- Average read length in 90% of samples ≥ 850 bases
– Minimum run time: ≤30 minutes

Other information

– Physical dimensions: 61 cm x 61 cm (122 cm open) x 72 cm (LxWxH)
– Weight: 82 kg
– Operating conditions:
+ Temperature 15°C–30°C
+ Humidity: 20-80%
+ Power supply: 100–240V (±10%); 50–60 Hz (±10%)
+ Electrophoresis voltage: Up to 20 kV

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– Up to 3 lasers Violet-Blue-Red (V-B-R) and fully activated with 13 colors

– Ability to measure 100 nm particles with violet laser

– Includes 13 band pass filters: 450/45, 525/40 (2), 585/42, 610/20 (2), 660/10 (2), 690/50, 712/25, 780/60 (3)

– For higher throughput applications an optional plate loader module is available

– Smart software in acquisition and analysis CytExpert