Certificate

Brand

Zymo Research

Product Type

Instrument

Highlights

Complete bisulfite conversion of GC-rich DNA in less than 3 hours

• Consolidated Workflow: DNA denaturation and bisulfite conversion process in one step.
• High Yield: Desulfonation and purification on a spin-column allows elution of bisulfite-converted DNA in as little as 10 µl.
• Suitable for Various Downstream Applications: Bisulfite-converted DNA can be robustly PCR amplified for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

Application

EZ DNA Methylation-Gold® Kits

  • Consolidated Workflow: DNA denaturation and bisulfite conversion process in one step.
  • High Yield: Desulfonation and purification on a spin-column allows elution of bisulfite-converted DNA in as little as 10 µl.
  • Suitable for Various Downstream Applications: Bisulfite-converted DNA can be robustly PCR amplified for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Specifications

Input 500 pg – 2 µg purified DNA
Conversion
Efficiency
> 99%
Elution Volume Spin Column: ≥ 10 µl
96-well: ≥ 15 µl
Magprep: ≥ 25 µl
DNA Recovery > 75%
Platform
Compatibility
Swift BiosciencesAccel-NGS®
Methyl-Seq DNA Library Kit
Agilent SureSelectXT Methyl-Seq
Target Enrichment System

Other information

▶ Supplier:

Zymo Research

▶ Order Information:

Product Cat. No. Size
EZ DNA Methylation-Gold® D5005
D5006
50 rxns
200 rxns
EZ-96 DNA Methylation-Gold® (shallow-well) D5007 2 x 96 rxns
EZ-96 DNA Methylation-Gold® (deep-well) D5008 2 x 96 rxns
EZ-96 EZ DNA Methylation-Gold® Magprep D5042 4 x 96 rxns

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