Thermo Fisher Scientific
Instrument
For Research Use Only
The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.
This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.
Format | 96-well Plate |
Thermal Accuracy | 0.25°C (35°C to 95°C after 3 min.) |
Sample Ramp Rate | ± 1.1°C⁄s |
Sensitivity | 1 Copy of RNase P gene (Human Genomic DNA) |
Temperature Range (Metric) | 4 to 100°C |
Reaction Volume Range | 20-100 µl |
Passive Reference Dye | ROX (Separate Tube), No ROX, ROX (Pre-mixed) |
Dynamic Range | 9 Logs |
Calibrated Dye | SYBR™ Green I, ROX™, Texas Red™, NED™, JOE™, Cy3™, Cy5™, VIC™, FAM™, TAMRA™ |
High-throughput Compatibility | Multiplexing |
For Use With (Equipment) | 7500 System |
Thermal Uniformity | 0.50°C (after 30 s) |
Reaction Speed | Standard |
Throughput | 4 to 5-96-well Plates per 8-hr day |
Optics | CCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters |
Thermal Cycling System | Peltier-based System |
Dimensions | Width 34 cm (13.39 in.), Depth 45 cm (17.72 in.), Height 49 cm (19.29 in.) |
External Computer | Dell Notebook D520 |
Software Compatibility | GeneMapper™ ID v3.2, 7500 System Software, HID Real Time PCR Analysis Software v1.1 |
Precision | 99.7% Confidence (5000 & 10000 Copies) |
Voltage | 120 V |
Electrical Requirements | 950 W |
Peak Block Ramp Rate | 2.5°C⁄s |
Weight | 75 lb. |
Built for Performance
The 7500 system for Human Identification includes SDS v.1.2.3 software which is validated to run Quantifiler™ Human DNA and Quantifiler™ Y Human Male DNA quantification kits. SDS v1.2.3 is a powerful, versatile software that includes plate set-up wizards and automated analysis tools that make data processing easier. Its advanced data viewing capabilities make analysis less time consuming. The instrument is a powerful, five-color platform that is calibrated for the broadest range of dyes available: FAM™⁄SYBR™ Green I, VIC™⁄JOE™, TAMRA™⁄CY3, ROX™⁄Texas Red, and CY5 dyes. Its specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets and its advanced multi-componenting algorithm minimizes spectral crosstalk making the instrument superior for multiplexing.
Complete Validated Solution
Applied Biosystems™ offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR™ STR kits, instrumentation, software, and consumables. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD. The system has been internally validated by Applied Biosystems™ following DAB⁄SWGDAM guidelines for use with Quantifiler™ Human Quantification System and Quantifiler™ Y Human Male DNA Quantification System making the 7500 system suitable for casework, paternity, and databasing laboratories.
EXPERIENCE MATTERS™
When you partner with Applied Biosystems™ you gain access to the extensive knowledge and experience of our forensic support team and training resources dedicated to Human Identification laboratories. Our HID University™ provides continuing education for DNA analysts, our support and Applications Scientists offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.
The GlobalFiler Express PCR Amplification Kit and Prep-n-Go Buffer (for buccal swabs) combines the features of our GlobalFiler Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer (for buccal swabs). The GlobalFiler Express kit is the first 6-dye, 24-locus STR kit that enables direct PCR amplification, eliminating the need to perform DNA extraction or purification steps for DNA database and other single-source reference samples. Prep-n-Go Buffer (for buccal swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.
The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.
The HID VeritiPro Thermal Cycler features a 96-well (0.2 mL) format with six independent temperature zones to provide precise temperature control and rapid temperature changes for conducting polymerase chain reactions (PCR). The HID VeritiPro Thermal Cycler features a powerful color touch screen and a USB port to simplify instrument setup and use.
Designed using capillary electrophoresis technology trusted by HID labs for over 25 years, the Applied Biosystems SeqStudio 8 Flex Genetic Analyzer for Human Identification with 8-capillary array delivers the flexibility and accuracy your work demands. Innovative capabilities, including a continuously accessible four-plate deck, can help you achieve reliable STR results efficiently. Features such as lab-friendly connectivity and easy, on-instrument training videos can help get you up and running quickly and minimize downtime.
Increased lab productivity
The SeqStudio 8 Flex Genetic Analyzer for HID is the genetic analyzer for HID fragment analysis and Sanger sequencing that delivers the high-quality data and reliable performance that forensic scientists expect, with design enhancements for improved flexibility, usability, connectivity, and serviceability.
Flexibility
• Accessibility—continuously load plates and reprioritize urgent samples without having to terminate the current run
• Increased walk-away capacity—four plate positions accommodating 8-tube strips and 96-well sample plates
• Work when and where you want—set up your plate and monitor run progress from anywhere using the Thermo Fisher Connect Platform.
Usability
• Designed for all levels of experience—simple one-button startup, autocalibration, and onscreen step by-step routine maintenance instructions and onboard self-help training videos
• Simplified capillary array installation—redesigned, more robust arrays with “click-and-slide” retractor tab and enhanced detection cell housing provide improved capillary protection, ease of handling, insertion, and positioning
• Compatibility with Applied Biosystems 3500 Series genetic analyzer buffers and polymers
• Easy inventory management with radio-frequency identification (RFID)–enabled tracking of consumables usage
• No desktop computer required—integrated touchscreen computer, intuitive software, walk-through wizards, and Plate Manager 2.1 Software for simplified plate setup and instrument operation
Connectivity
Integrate the communication technologies and security options of today to propel laboratory efficiencies of tomorrow.
• Connect your way—integrate your instrument via local area network (LAN), Wi-Fi, USB ports, and LIMS compatibility
• Confidently walk away—monitor your run and data via the Connect Platform from your mobile phone, tablet, laptop, or remote desktop
• Data integrity and compliance—optional security, audit, and electronic signature (SAE) software for an electronic chain of custody
Serviceability
Applied Biosystems instrument innovation continues with additionally included digital service ecosystem for remote troubleshooting.*
• Reduced service visits—70% of issues remotely resolved
• Reduced instrument downtime—remotely resolve issues in minutes, not days, using onboard Smart Help and Remote Support features
• Real-time audio/video collaboration—the Remote Support feature brings our technicians into your laboratory using cutting-edge, security-minded digital tools
• Secure transfer of instrument performance data—send log and run files directly from the instrument with the push of a button using Smart Help
*Connectivity required for Smart Help and Remote Monitoring
Upgrade capabilities when you need them
The SeqStudio 8 Flex Genetic Analyzer can be seamlessly upgraded to a SeqStudio 24 Flex Genetic Analyzer by a field service engineer to provide additional throughput as your workload increases.
Designed using capillary electrophoresis technology trusted by HID labs for over 25 years, the Applied Biosystems SeqStudio 24 Flex Genetic Analyzer for Human Identification with 24-capillary array delivers the flexibility and accuracy your work demands. Innovative capabilities, including a continuously accessible four-plate deck, can help you achieve reliable STR results efficiently. Features such as lab-friendly connectivity and easy, on-instrument training videos can help get you up and running quickly and minimize downtime.
Increased lab productivity
The SeqStudio 24 Flex Genetic Analyzer for HID is the genetic analyzer for HID fragment analysis and Sanger sequencing that delivers the high-quality data and reliable performance that forensic scientists expect, with design enhancements for improved flexibility, usability, connectivity, and serviceability.
Flexibility
• Accessibility—continuously load plates and reprioritize urgent samples without having to terminate the current run
• Increased walk-away capacity—four plate positions accommodating 8-tube strips and 96-well sample plates
• Work when and where you want—set up your plate and monitor run progress from anywhere using the Thermo Fisher Connect Platform.
Usability
• Designed for all levels of experience—simple one-button startup, autocalibration, and onscreen step by-step routine maintenance instructions and onboard self-help training videos
• Simplified capillary array installation—redesigned, more robust arrays with “click-and-slide” retractor tab and enhanced detection cell housing provide improved capillary protection, ease of handling, insertion, and positioning • Compatibility with Applied Biosystems 3500 Series genetic analyzer buffers and polymers
• Easy inventory management with radio-frequency identification (RFID)–enabled tracking of consumables usage
• No desktop computer required—integrated touchscreen computer, intuitive software, walk-through wizards, and Plate Manager 2.1 Software for simplified plate setup and instrument operation
Connectivity
Integrate the communication technologies and security options of today to propel laboratory efficiencies of tomorrow.
• Connect your way—integrate your instrument via local area network (LAN), Wi-Fi, USB ports, and LIMS compatibility
• Confidently walk away—monitor your run and data via the Connect Platform from your mobile phone, tablet, laptop, or remote desktop
• Data integrity and compliance—optional security, audit, and electronic signature (SAE) software for an electronic chain of custody
Serviceability
Applied Biosystems instrument innovation continues with additionally included digital service ecosystem for remote troubleshooting.*
• Reduced service visits—70% of issues remotely resolved
• Reduced instrument downtime—remotely resolve issues in minutes, not days, using onboard Smart Help and Remote Support features
• Real-time audio/video collaboration—the Remote Support feature brings our technicians into your laboratory using cutting-edge, security-minded digital tools
• Secure transfer of instrument performance data—send log and run files directly from the instrument with the push of a button using Smart Help
*Connectivity required for Smart Help and Remote Monitoring
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