Certificate

Brand

illumina

Product Type

Instrument

Highlights

Fast, flexible, high-throughput benchtop sequencers enable whole-genome, transcriptome, and targeted resequencing applications with the accuracy of Illumina SBS technology.

Model: Nextseq 550
Manufacturer: Illumina/USA
– Is the only sequencing system capable of deploying applications running sequencing and reading microarray biochips
– New generation DNA sequencing system using synthetic sequencing technology: Sequencing by Synthesis – SBS
– The whole process of gene sequencing is conducted on the surface of flow cells, convenient to use and easy to manipulate.

Application

– Detecting genetic diseases, serving for prenatal diagnosis, using Beadchip array technology

– Screening for abnormal chromosome number of chromosomes 13, 18, 21, X, Y with fetuses from 10 weeks of age for natural pregnancies, 8 weeks of age for pregnancies by In vitro Fertilization

– Sequencing of whole human genome, whole small genome, whole exome, transcriptome and studying gene expression

– Targeted gene sequencing. Able to clearly detect mutations, serving in cancer diagnosis and targeted treatment

– Sequencing 16S RNA applied in Metagenomics analysis, environmental analysis, identification of bacterial pathogens

– mRNA sequencing; small RNA in research

Specifications

– A high-powered sequencing system capable of sequencing an entire human genome (with 30X data depth) in one run.

– Sequencing up to 16 exome systems, 20 transcriptome systems and 40 gene expression systems in one run.

– Sequencing up to 15 non-invasive prenatal testing (NIPT) samples.

– Genome reading capacity: 16-120 Gb in 1 run, suitable for many applications.

– The number of paragraphs reading after filtering reached 130 – 800 million paragraphs in 1 run.

NextSeq 550 System High-Output Kit* NextSeq 550 System Mid-Output Kit*
Read Length Total Time Output Read Length Total Time Output
2 × 150 bp 29 hrs 100–120 Gb 2 × 150 bp 26 hrs 32.5–39 Gb
2 × 75 bp 18 hrs 50–60 Gb 2 × 75 bp 15 hrs 16.25–19.5 Gb
1 × 75 bp 11 hrs 25–30 Gb

 

Read Length NextSeq 550 System High-Output Kit NextSeq 550 System Mid-Output Kit
2 x 150 bp > 75% bases higher than Q30 at 2 × 150 bp > 75% bases higher than Q30 at 2 × 150 bp
2 x 75 bp > 80% bases higher than Q30 at 2 × 75 bp > 80% bases higher than Q30 at 2 × 75 bp
1 x 75 bp > 80% bases higher than Q30 at 1 × 75 bp

*Q30 data quality equivalent to 99.9% accuracy

– Fully automatic sequencing process, software integration intelligent control:

Normal sequencing processes from sample separation to result analysis within 30 hours
Especially with the NIPT screening process, DNA sequencing is isolated from maternal blood for analysis and results within 24 hours.

In addition, the NextSeq 550 system has built-in microarray readings:

1. Infinium Human CytoSNP-12 BeadChip: Research on cell genetics, population genetics, lineage classification based on SNP technology. Each BeadChip was scanned in 40 minutes, scanning each sample took 3.3 minutes.

2. Infinium CytoSNP-850k BeadChip: Research on cell genetics, population genetics, lineage classification with high resolution. Each BeadChip is scanned in 40 minutes, each sample takes 5 minutes

3. Infinium HumanKaryomap-12 BeadChip: Diagnosis of embryo genetic diseases (PGD) when performing assisted reproductive measures (Invitro fertilization – IVF). Each BeadChip was scanned in 40 minutes, scanning each sample took 3.3 minutes.

Details at: https://www.illumina.com/systems/sequencing-platforms/nextseq/specifications.html

Other information

Supplier

illumina

Related Products

EZ DNA Methylation-Gold® Kits

• Consolidated Workflow: DNA denaturation and bisulfite conversion process in one step.
• High Yield: Desulfonation and purification on a spin-column allows elution of bisulfite-converted DNA in as little as 10 µl.
• Suitable for Various Downstream Applications: Bisulfite-converted DNA can be robustly PCR amplified for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

Solution: NgeneBio – SOLIDaccuTest™

SOLIDaccuTest™ is an excellent tool to explorer variants associated with solid tumors using a comprehensive method of next-generation sequencing (NGS). It reflects the latest research trends and is optimized for the medical purpose by selecting essential genes of solid tumors including lung, colon, breast, skin brain, gastric, ovarian cancers, etc.

 

BRCAaccuTest™ & BRCAaccuTest™PLUS

BRCAaccuTest™ PLUS with CE-IVD certificate for HBOC Breast/Ovarian Cancer Test

Next Generation Sequencing System – NovaSeq 6000

Model: NovaSeq 6000
Manufacturer: Illumina/USA
– Scalable platform
Match data output, time to results, and price per sample to study needs
– Flexible performance
Configure sequencing method, flow cell type, and read length to support a broad range of applications
– Streamlined operation
Increase lab efficiency with a simplified workflow and reduced hands-on time

Zymot multi 850uL

Zymot with simple operation process, helps to collect high-quality sperm, improve the efficiency of Assisted Reproduction (IVF, IUI, ICSI).

NextSeq 1000 sequencing system

Product Information

Model: NextSeq 1000

Manufacturer: Illumina

Origin: Singapore/United States

Launching year: 01/2020

Basic Yellow 40

Product Information

Model: Basic Yellow

Manufacturer: Lynn Peavey

Origin: United States

Rhodamine 6G

Product Information

Model: Rhodamine 6g

Manufacturer: Lynn Peavey

Origin: United States

Next-Generation Sequencing System – NovaSeq 6000

Model: NovaSeq 6000
Manufacturer: Illumina/USA
– Scalable platform
Match data output, time to results, and price per sample to study needs
– Flexible performance
Configure sequencing method, flow cell type, and read length to support a broad range of applications
– Streamlined operation
Increase lab efficiency with a simplified workflow and reduced hands-on time

Next-Generation Sequencing System – NovaSeq 6000

Model: NovaSeq 6000
Manufacturer: Illumina/USA
– Scalable platform
Match data output, time to results, and price per sample to study needs
– Flexible performance
Configure sequencing method, flow cell type, and read length to support a broad range of applications
– Streamlined operation
Increase lab efficiency with a simplified workflow and reduced hands-on time