Next Generation Sequencing System - NextSeq 550 - BIOMEDIC JSC

Home

Products & Applications

Obstetrics & Gynecology

Next Generation Sequencing System – NextSeq 550

Certificate

Brand

Illumina

Product Type

Instrument

Highlights

Fast, flexible, high-throughput benchtop sequencers enable whole-genome, transcriptome, and targeted resequencing applications with the accuracy of Illumina SBS technology.

Model: Nextseq 550
Manufacturer: Illumina/USA
– Is the only sequencing system capable of deploying applications running sequencing and reading microarray biochips
– New generation DNA sequencing system using synthetic sequencing technology: Sequencing by Synthesis – SBS
– The whole process of gene sequencing is conducted on the surface of flow cells, convenient to use and easy to manipulate.

Application

– Detecting genetic diseases, serving for prenatal diagnosis, using Beadchip array technology

– Screening for abnormal chromosome number of chromosomes 13, 18, 21, X, Y with fetuses from 10 weeks of age for natural pregnancies, 8 weeks of age for pregnancies by In vitro Fertilization

– Sequencing of whole human genome, whole small genome, whole exome, transcriptome and studying gene expression

– Targeted gene sequencing. Able to clearly detect mutations, serving in cancer diagnosis and targeted treatment

– Sequencing 16S RNA applied in Metagenomics analysis, environmental analysis, identification of bacterial pathogens

– mRNA sequencing; small RNA in research

Specifications

– A high-powered sequencing system capable of sequencing an entire human genome (with 30X data depth) in one run.

– Sequencing up to 16 exome systems, 20 transcriptome systems and 40 gene expression systems in one run.

– Sequencing up to 15 non-invasive prenatal testing (NIPT) samples.

– Genome reading capacity: 16-120 Gb in 1 run, suitable for many applications.

– The number of paragraphs reading after filtering reached 130 – 800 million paragraphs in 1 run.

NextSeq 550 System High-Output Kit*			NextSeq 550 System Mid-Output Kit*
Read Length	Total Time^†	Output	Read Length	Total Time^†	Output
2 × 150 bp	29 hrs	100–120 Gb	2 × 150 bp	26 hrs	32.5–39 Gb
2 × 75 bp	18 hrs	50–60 Gb	2 × 75 bp	15 hrs	16.25–19.5 Gb
1 × 75 bp	11 hrs	25–30 Gb

Read Length	NextSeq 550 System High-Output Kit	NextSeq 550 System Mid-Output Kit
2 x 150 bp	> 75% bases higher than Q30 at 2 × 150 bp	> 75% bases higher than Q30 at 2 × 150 bp
2 x 75 bp	> 80% bases higher than Q30 at 2 × 75 bp	> 80% bases higher than Q30 at 2 × 75 bp
1 x 75 bp	> 80% bases higher than Q30 at 1 × 75 bp

*Q30 data quality equivalent to 99.9% accuracy

– Fully automatic sequencing process, software integration intelligent control:

Normal sequencing processes from sample separation to result analysis within 30 hours
Especially with the NIPT screening process, DNA sequencing is isolated from maternal blood for analysis and results within 24 hours.

In addition, the NextSeq 550 system has built-in microarray readings:

1. Infinium Human CytoSNP-12 BeadChip: Research on cell genetics, population genetics, lineage classification based on SNP technology. Each BeadChip was scanned in 40 minutes, scanning each sample took 3.3 minutes.

2. Infinium CytoSNP-850k BeadChip: Research on cell genetics, population genetics, lineage classification with high resolution. Each BeadChip is scanned in 40 minutes, each sample takes 5 minutes

3. Infinium HumanKaryomap-12 BeadChip: Diagnosis of embryo genetic diseases (PGD) when performing assisted reproductive measures (Invitro fertilization – IVF). Each BeadChip was scanned in 40 minutes, scanning each sample took 3.3 minutes.

Details at: https://www.illumina.com/systems/sequencing-platforms/nextseq/specifications.html

Other information

Supplier

illumina

Related Products

GlobalFiler™ IQC PCR Amplification Kit

Label or Dye 6-FAM, LIZ, NED, SID, TAZ, VIC Product Line GlobalFiler™ Product Type PCR Amplification Kit Shipping Condition Dry Ice Target Loci Amelogenin, CSF1PO, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D1S1656, D21S11, D22S1045, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, DYS391, FGA, IQC-Large, IQC-Small, SE33, TH01, TPOX, Y indel, vWA

GlobalFiler™ Express PCR Amplification Kit

The GlobalFiler Express PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler Express kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler PCR Amplification Kit.

EZ DNA Methylation-Gold® Kits

• Consolidated Workflow: DNA denaturation and bisulfite conversion process in one step.
• High Yield: Desulfonation and purification on a spin-column allows elution of bisulfite-converted DNA in as little as 10 µl.
• Suitable for Various Downstream Applications: Bisulfite-converted DNA can be robustly PCR amplified for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

Next Generation Sequencing System – NovaSeq X Series

NovaSeq X also significantly reduces waste and environmental impact, reflecting Illumina’s commitment to using its technology to support the health of people and the planet. NovaSeq X features a 90% reduction in packaging waste and weight and 50% reduction in plastic usage compared to NovaSeq 6000.

Zymot multi 3mL

Zymot with simple operation process, helps to collect high-quality sperm, improve the efficiency of Assisted Reproduction (IVF, IUI, ICSI).

Zymot multi 850uL

Zymot with simple operation process, helps to collect high-quality sperm, improve the efficiency of Assisted Reproduction (IVF, IUI, ICSI).

Next-Generation Sequencing System – NextSeq 2000

The NextSeq 2000 Sequencing System uses patterned flow cells similar to those that power the NovaSeq™ 6000 System. The result is a highly flexible and scalable benchtop system that offers the highest cluster density flow cell of any on-market NGS system to date, driving down the cost per gigabase (Gb) of the sequencing run.

PANARealTyperTM HPV Screening Kit

The PANA RealTyper™ HPV Screening Kit is a CE marked diagnostic device in accordance with the European Union in vitro Diagnostic Medical Device Directive 98/79/EC

CytoFLEX

Model: CytoFLEX
Manufacturer: Beckman Coulter Life Sciences/ USA

The CytoFLEX Flow Cytometer is the most innovative flow cytometer system of Beckman Coulter. With compact design and high efficiency in work, CytoFlex provides and easy to use system with the impressive performance you need. Our system allows the researchers just focus on the science with no worry about instrument. Its superior sensitivity and resolution throughout all configurations give it the edge over other cytometry systems four times its size.

– Up to 3 lasers Violet-Blue-Red (V-B-R) and fully activated with 13 colors

– Ability to measure 100 nm particles with violet laser

– Includes 13 band pass filters: 450/45, 525/40 (2), 585/42, 610/20 (2), 660/10 (2), 690/50, 712/25, 780/60 (3)

– For higher throughput applications an optional plate loader module is available

– Smart software in acquisition and analysis CytExpert

Next Generation Sequencing System – iSeq 100

Model: iSeq 100
Manufacturer: Illumina/USA
Illumina’s smallest next-generation DNA sequencing system, with small, fast and efficient sequencing throughput, suitable for all labs
– Fast data generation: Suitable for small projects, on a dedicated device, low throughput with fast turnaround times
– Convenient operation: Control the sequencing process from start to finish and ensure independent sequencing instead of outsourcing.

Obstetrics & Gynecology

Oncology

Infectious diseases

Forensics

Obstetrics & Gynecology

Oncology

Infectious diseases

Forensics