Next Generation Sequencing System - NextSeq 550 - BIOMEDIC JSC

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Next Generation Sequencing System – NextSeq 550

Certificate

Brand

Illumina

Product Type

Instrument

Highlights

Fast, flexible, high-throughput benchtop sequencers enable whole-genome, transcriptome, and targeted resequencing applications with the accuracy of Illumina SBS technology.

Model: Nextseq 550
Manufacturer: Illumina/USA
– Is the only sequencing system capable of deploying applications running sequencing and reading microarray biochips
– New generation DNA sequencing system using synthetic sequencing technology: Sequencing by Synthesis – SBS
– The whole process of gene sequencing is conducted on the surface of flow cells, convenient to use and easy to manipulate.

Application

– Detecting genetic diseases, serving for prenatal diagnosis, using Beadchip array technology

– Screening for abnormal chromosome number of chromosomes 13, 18, 21, X, Y with fetuses from 10 weeks of age for natural pregnancies, 8 weeks of age for pregnancies by In vitro Fertilization

– Sequencing of whole human genome, whole small genome, whole exome, transcriptome and studying gene expression

– Targeted gene sequencing. Able to clearly detect mutations, serving in cancer diagnosis and targeted treatment

– Sequencing 16S RNA applied in Metagenomics analysis, environmental analysis, identification of bacterial pathogens

– mRNA sequencing; small RNA in research

Specifications

– A high-powered sequencing system capable of sequencing an entire human genome (with 30X data depth) in one run.

– Sequencing up to 16 exome systems, 20 transcriptome systems and 40 gene expression systems in one run.

– Sequencing up to 15 non-invasive prenatal testing (NIPT) samples.

– Genome reading capacity: 16-120 Gb in 1 run, suitable for many applications.

– The number of paragraphs reading after filtering reached 130 – 800 million paragraphs in 1 run.

NextSeq 550 System High-Output Kit*			NextSeq 550 System Mid-Output Kit*
Read Length	Total Time^†	Output	Read Length	Total Time^†	Output
2 × 150 bp	29 hrs	100–120 Gb	2 × 150 bp	26 hrs	32.5–39 Gb
2 × 75 bp	18 hrs	50–60 Gb	2 × 75 bp	15 hrs	16.25–19.5 Gb
1 × 75 bp	11 hrs	25–30 Gb

Read Length	NextSeq 550 System High-Output Kit	NextSeq 550 System Mid-Output Kit
2 x 150 bp	> 75% bases higher than Q30 at 2 × 150 bp	> 75% bases higher than Q30 at 2 × 150 bp
2 x 75 bp	> 80% bases higher than Q30 at 2 × 75 bp	> 80% bases higher than Q30 at 2 × 75 bp
1 x 75 bp	> 80% bases higher than Q30 at 1 × 75 bp

*Q30 data quality equivalent to 99.9% accuracy

– Fully automatic sequencing process, software integration intelligent control:

Normal sequencing processes from sample separation to result analysis within 30 hours
Especially with the NIPT screening process, DNA sequencing is isolated from maternal blood for analysis and results within 24 hours.

In addition, the NextSeq 550 system has built-in microarray readings:

1. Infinium Human CytoSNP-12 BeadChip: Research on cell genetics, population genetics, lineage classification based on SNP technology. Each BeadChip was scanned in 40 minutes, scanning each sample took 3.3 minutes.

2. Infinium CytoSNP-850k BeadChip: Research on cell genetics, population genetics, lineage classification with high resolution. Each BeadChip is scanned in 40 minutes, each sample takes 5 minutes

3. Infinium HumanKaryomap-12 BeadChip: Diagnosis of embryo genetic diseases (PGD) when performing assisted reproductive measures (Invitro fertilization – IVF). Each BeadChip was scanned in 40 minutes, scanning each sample took 3.3 minutes.

Details at: https://www.illumina.com/systems/sequencing-platforms/nextseq/specifications.html

Other information

Supplier

illumina

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– Ability to measure 100 nm particles with violet laser

– Includes 13 band pass filters: 450/45, 525/40 (2), 585/42, 610/20 (2), 660/10 (2), 690/50, 712/25, 780/60 (3)

– For higher throughput applications an optional plate loader module is available

– Smart software in acquisition and analysis CytExpert

Obstetrics & Gynecology

Oncology

Infectious diseases

Forensics

Obstetrics & Gynecology

Oncology

Infectious diseases

Forensics