Next Generation Sequencing System - NextSeq 550 - BIOMEDIC JSC

Home

Products & Applications

Obstetrics & Gynecology

Next Generation Sequencing System – NextSeq 550

Certificate

Brand

Illumina

Product Type

Instrument

Highlights

Fast, flexible, high-throughput benchtop sequencers enable whole-genome, transcriptome, and targeted resequencing applications with the accuracy of Illumina SBS technology.

Model: Nextseq 550
Manufacturer: Illumina/USA
– Is the only sequencing system capable of deploying applications running sequencing and reading microarray biochips
– New generation DNA sequencing system using synthetic sequencing technology: Sequencing by Synthesis – SBS
– The whole process of gene sequencing is conducted on the surface of flow cells, convenient to use and easy to manipulate.

Application

– Detecting genetic diseases, serving for prenatal diagnosis, using Beadchip array technology

– Screening for abnormal chromosome number of chromosomes 13, 18, 21, X, Y with fetuses from 10 weeks of age for natural pregnancies, 8 weeks of age for pregnancies by In vitro Fertilization

– Sequencing of whole human genome, whole small genome, whole exome, transcriptome and studying gene expression

– Targeted gene sequencing. Able to clearly detect mutations, serving in cancer diagnosis and targeted treatment

– Sequencing 16S RNA applied in Metagenomics analysis, environmental analysis, identification of bacterial pathogens

– mRNA sequencing; small RNA in research

Specifications

– A high-powered sequencing system capable of sequencing an entire human genome (with 30X data depth) in one run.

– Sequencing up to 16 exome systems, 20 transcriptome systems and 40 gene expression systems in one run.

– Sequencing up to 15 non-invasive prenatal testing (NIPT) samples.

– Genome reading capacity: 16-120 Gb in 1 run, suitable for many applications.

– The number of paragraphs reading after filtering reached 130 – 800 million paragraphs in 1 run.

NextSeq 550 System High-Output Kit*			NextSeq 550 System Mid-Output Kit*
Read Length	Total Time^†	Output	Read Length	Total Time^†	Output
2 × 150 bp	29 hrs	100–120 Gb	2 × 150 bp	26 hrs	32.5–39 Gb
2 × 75 bp	18 hrs	50–60 Gb	2 × 75 bp	15 hrs	16.25–19.5 Gb
1 × 75 bp	11 hrs	25–30 Gb

Read Length	NextSeq 550 System High-Output Kit	NextSeq 550 System Mid-Output Kit
2 x 150 bp	> 75% bases higher than Q30 at 2 × 150 bp	> 75% bases higher than Q30 at 2 × 150 bp
2 x 75 bp	> 80% bases higher than Q30 at 2 × 75 bp	> 80% bases higher than Q30 at 2 × 75 bp
1 x 75 bp	> 80% bases higher than Q30 at 1 × 75 bp

*Q30 data quality equivalent to 99.9% accuracy

– Fully automatic sequencing process, software integration intelligent control:

Normal sequencing processes from sample separation to result analysis within 30 hours
Especially with the NIPT screening process, DNA sequencing is isolated from maternal blood for analysis and results within 24 hours.

In addition, the NextSeq 550 system has built-in microarray readings:

1. Infinium Human CytoSNP-12 BeadChip: Research on cell genetics, population genetics, lineage classification based on SNP technology. Each BeadChip was scanned in 40 minutes, scanning each sample took 3.3 minutes.

2. Infinium CytoSNP-850k BeadChip: Research on cell genetics, population genetics, lineage classification with high resolution. Each BeadChip is scanned in 40 minutes, each sample takes 5 minutes

3. Infinium HumanKaryomap-12 BeadChip: Diagnosis of embryo genetic diseases (PGD) when performing assisted reproductive measures (Invitro fertilization – IVF). Each BeadChip was scanned in 40 minutes, scanning each sample took 3.3 minutes.

Details at: https://www.illumina.com/systems/sequencing-platforms/nextseq/specifications.html

Other information

Supplier

illumina

Related Products

SeqStudio™ 24 Flex Genetic Analyzer

The Applied Biosystems SeqStudio 24 Flex Genetic Analyzer offers design improvements and technological advances to the capillary electrophoresis (CE) workflow. It delivers gold-standard Sanger sequencing and fragment analysis data quality with increased flexibility, easier operation, and cloud connectivity to enable researchers to manage their workloads and work/life balance effectively. Designed for ultimate ease-of-use, this mid-throughput system offers high quality, excellent reliability, and an optimal user experience for researchers who want to work smarter in the lab.

SeqStudio™ 8 Flex Genetic Analyzer

The Applied Biosystems SeqStudio 8 Flex Genetic Analyzer offers design improvements and technological advances to the capillary electrophoresis (CE) workflow. It delivers gold-standard Sanger sequencing and fragment analysis data quality with increased flexibility, easier operation, and cloud connectivity to enable researchers to manage their workloads and work/life balance effectively. Designed for ultimate ease-of-use, this mid-throughput system offers high quality, excellent reliability, and an optimal user experience for researchers who want to work smarter in the lab.

QuantStudio™ 7 Pro Real-Time PCR System, TaqMan™ Array Card, desktop

The Applied Biosystems QuantStudio 7 Pro Real-Time PCR System adds innovative smart features to the real-time PCR (qPCR) workflow, all in a compact footprint. With facial authentication, voice commands, Smart Help, multiplex capability, motorized interchangeable blocks, simplified workflow, intuitive software, automation capabilities, and large capacitive touch-screen interface, the QuantStudio 7 Pro system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Designed for ultimate ease-of-use, the system offers high quality, high throughput, excellent reliability, and an optimal user experience for researchers who want to work smarter in the lab.

EmbryoMap

EmbryoMap for labs assessing preimplantation embryo biopsy samples

Through evolution and innovation, the EmbryoMap solution has been analytically validated for detection of whole- and sub-chromosomal imbalances with highly reproducible copy-number, using cell line reference material and embryo biopsy samples

Samples are prepared for sequencing using the EmbryoMap Sample Prep Kit, sequenced on the Illumina MiSeq Sequencing System and data analysis and reporting performed using eMap software

Infinium KaryoMap v2

The kit contains BeadChips and reagents to perform the KaryoMap v2 assay sufficient for 16 samples.

Disclaimer: This product is labelled for Research Use Only – Not for use in diagnostic procedures.

Next Generation Sequencing System – NovaSeq X Series

NovaSeq X also significantly reduces waste and environmental impact, reflecting Illumina’s commitment to using its technology to support the health of people and the planet. NovaSeq X features a 90% reduction in packaging waste and weight and 50% reduction in plastic usage compared to NovaSeq 6000.

Next-Generation Sequencing System – NextSeq 2000

The NextSeq 2000 Sequencing System uses patterned flow cells similar to those that power the NovaSeq™ 6000 System. The result is a highly flexible and scalable benchtop system that offers the highest cluster density flow cell of any on-market NGS system to date, driving down the cost per gigabase (Gb) of the sequencing run.

NextSeq 1000 sequencing system

Product Information

Model: NextSeq 1000

Manufacturer: Illumina

Origin: Singapore/United States

Launching year: 01/2020

Rhodamine 6G

Product Information

Model: Rhodamine 6g

Manufacturer: Lynn Peavey

Origin: United States

DxFLEX

DxFLEX is a new clinical flow cytometry platform derived from the successful CytoFLEX. The advanced sensitivity and intuitive software DxFLEX makes flow cytometry routine for both novice and expert flow cytometry technicians and promotes standardization. Functionality in the autoloader facilitates accurate results and sample tracking.

Obstetrics & Gynecology

Oncology

Infectious diseases

Forensics

Obstetrics & Gynecology

Oncology

Infectious diseases

Forensics