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TruSight Tumor 170 Kit includes library preparation reagent set and uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 170 genes that are commonly related to mutations in solid tumors and somatic variants.

Thông số kỹ thuật

Specifications:

Assay Time Total 32 hours
Sequence Run Time 24 hours on NextSeq, 27 hours on HiSeq 2500
Technology Next-Generation Sequencing (NGS)
Input Quantity 40 ng DNA, 40 ng RNA
Gene Sequencing 2 × 101 cycles
Minimum insert size 79 bp DNA, 63 bp RNA
Amplicon Size FFPE, low input
Specialised Sample Types MiniSeq, MiSeq, MiSeqDx in Reseach Mode
NGS System Compatibility 8 samples/run on NextSeq

6 runs on HiSeq 2500

Sensitivity and specificity > 95%
Variant Frequency ≤5%
Variant Class Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Polymorphisms (SNPs), Somatic Variants, Structural Variants

 

Sản phẩm liên quan

Basic Yellow 40

Thông tin sản phẩm

Model: Basic Yellow

Hãng sản xuất: Lynn Peavey

Xuất xứ: Mỹ

Biomek i7 Automated Workstation

Designed for your evolving workflow priorities Representing the best of what made Biomek an industry leading liquid handler—combined with enhancements suggested by customers around the globe—the Biomek i7 Automated Workstation has been designed to optimize workflows and increase walk-away in mid- to high-throughput labs.

Hệ thống tự động Biomek i7

Thiết kế cho các quy trình phát triển của bạn Hệ thống xử lý chất lỏng tốt nhất, dẫn đầu thị trường – được kết nối với các phần bổ sung theo đề xuất từ các khách hàng trên toàn cầu

Hệ thống tự động Biomek i7 được thiết kế để tối ưu quy trình và tăng thời gian “walk-away” ở các phòng lab công suất trung bình đến lớn.

Biomek i5 Automated Workstation

Optimize dependability and walk-away time. The Biomek i5 is the newest addition to our liquid handling portfolio representing the best of what has made Biomek an industry leading brand—combined with enhancements suggested by customers around the globe.

– Ideal for medium- to high-throughput workflows

– 25 deck positions

– 0.5 µL-1,000 µL (Multichannel) or 0.5 µL-5,000 µL (Span-8) pipetting volume ranges

– Single Multichannel head (96/384) or Span-8 pipetting with gripper

Quick-DNA

Tách chiết DNA từ tế bào và máu toàn phần:
– Dễ dàng tách chiết DNA từ máu toàn phần, buffy coat, mẫu tăm bông, hoặc mẫu nuôi cấy tế bào.
– Quy trình k cần sử dụng Proteinase K và các chất biến tính cho mẫu dịch sinh hoặc và mẫu nuôi cấy tế bào.

Quick-RNA Viral

Viral DNA and RNA from all biological fluids:
– Quick recovery of viral RNA from plasma, serum and other samples.
– Omits the use of organic denaturants and proteases.
– High-quality viral RNA is ready for RT-PCR, sequencing, etc.

DNA Sequencing Clean-Up

Reusable and simple purification kit for preparing sequence-ready DNA:
– Complete elimination of “Dye Blobs” for high quality Phred scores and long read lengths.
– Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps.
– Reusable!

DNA Clean & Concentrator (DCC)

Clean-up PCR and other enzymatic reactions in as little as 2 minutes:
– Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution in as little as 2 minutes with 0 μl wash residue carryover.
– Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
– Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

DNA Clean & Concentrator (DCC)

Clean-up PCR and other enzymatic reactions in as little as 2 minutes:
– Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution in as little as 2 minutes with 0 μl wash residue carryover.
– Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
– Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

Quick-DNA

Rapid purification of DNA from cells and whole blood:
– Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
– Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples. are fracture resistant and chemically insert.

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