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Hệ thống giải trình tự thế hệ mới NextSeq 2000

TruSight Tumor 170 Kit includes library preparation reagent set and uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 170 genes that are commonly related to mutations in solid tumors and somatic variants.

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Specifications:

Assay Time Total 32 hours
Sequence Run Time 24 hours on NextSeq, 27 hours on HiSeq 2500
Technology Next-Generation Sequencing (NGS)
Input Quantity 40 ng DNA, 40 ng RNA
Gene Sequencing 2 × 101 cycles
Minimum insert size 79 bp DNA, 63 bp RNA
Amplicon Size FFPE, low input
Specialised Sample Types MiniSeq, MiSeq, MiSeqDx in Reseach Mode
NGS System Compatibility 8 samples/run on NextSeq

6 runs on HiSeq 2500
Sensitivity and specificity > 95%
Variant Frequency ≤5%
Variant Class Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Polymorphisms (SNPs), Somatic Variants, Structural Variants

 

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Reusable and simple purification kit for preparing sequence-ready DNA:
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– Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps.
– Reusable!

DNA Clean & Concentrator (DCC)

Clean-up PCR and other enzymatic reactions in as little as 2 minutes:
– Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution in as little as 2 minutes with 0 μl wash residue carryover.
– Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
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Easily isolate DNA from tough-to-lyse fungi and bacteria:
– Simple, efficient isolation of DNA from all types of tough-to-lyse fungi and bacteria in minutes.
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Hệ thống giải trình tự thế hệ mới NextSeq 2000