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TruSight Tumor 170 Kit includes library preparation reagent set and uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 170 genes that are commonly related to mutations in solid tumors and somatic variants.

Thông số kỹ thuật

Specifications:

Assay Time Total 32 hours
Sequence Run Time 24 hours on NextSeq, 27 hours on HiSeq 2500
Technology Next-Generation Sequencing (NGS)
Input Quantity 40 ng DNA, 40 ng RNA
Gene Sequencing 2 × 101 cycles
Minimum insert size 79 bp DNA, 63 bp RNA
Amplicon Size FFPE, low input
Specialised Sample Types MiniSeq, MiSeq, MiSeqDx in Reseach Mode
NGS System Compatibility 8 samples/run on NextSeq

6 runs on HiSeq 2500

Sensitivity and specificity > 95%
Variant Frequency ≤5%
Variant Class Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Polymorphisms (SNPs), Somatic Variants, Structural Variants

 

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