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Hệ thống giải trình tự thế hệ mới NextSeq 2000

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GeneProof Hepatitis B Virus (HBV) PCR Kit applies PCR technology to detect DNA of HBV virus in patient samples. The kit has CE-IVD certification for in vitro Diagnostic Use.

Ứng dụng
Thông số kỹ thuật

▶ Detection ability

  • Specificity: HBV genotype A-H, pre-core mutants HBV (HBeAg negative), 100%
  • Sensitivity (LOD): reaches up 36.979 IU/ml  with probability of 95% (on HBV NIBSC 02/264/01 using manual extraction GeneProof PathogenFree DNA Isolation Kit.

▶ Technology

The HBV detection consists in amplification of a specific conservative DNA sequence of an open reading frame X (ORFx) and in measurement of fluorescence increase. The HBV presence is indicated by the FAM fluorophore fluorescence growth. The detection kit takes advantage of the ”hot start” technology, minimizing non-specific reactions and assuring maximum sensitivity.

– Internal Standard (IS) is included in the PCR kit, controlling the possible inhibition of the PCR and the DNA  extraction process quality (ISEX version

– Ready to Use MasterMix contains uracil DNA glycosylase (UDG), eliminating possible contamination of the PCR  with amplification products

▶  Validated Specimen: plasma, serum

▶ Certification: CE-IVD for in vitro Diagnostic Use

▶ Order information

Product Name

Cat. No

Package

GeneProof Hepatitis B Virus (HBV) PCR Kit

HBV/ISEX/025

HBV/ISEX/050

HBV/ISEX/100

25 reactions

50 reactions

100 reactions

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Hệ thống giải trình tự thế hệ mới NextSeq 2000