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The Nextera DNA Flex Library Prep Kit provides library preparation reagent for DNA sequencing on Next Generation Sequencing system of Illumina.

Thông số kỹ thuật

Parameters
Assay Time ~ 3.5-4 hours
Hands-on Time 1-1.5 hours
Specialised Sample Types gDNA, Blood, Saliva, PCR Amplicons, Plasmids, Dried Blood Spots, Low Input Samples
DNA Input Type ·   Small Genomes: 1-500 ng DNA,

·   Large Genomes: 100-500 ng

Technology Human Whole Genome, Small Whole Genome, Large Whole Genome of any species including Human Genome
Samples/run Maximum 96 samples
Insert Size 300-350 bp
Variant Class Chromosomal Abnormalities, Copy Number Variants (CNVs), Gene Fusions, Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants, Structural Variants
NGS System Compatibility HiSeq 2500HiSeq 3000HiSeq 4000HiSeq X FiveHiSeq X TenMiniSeqMiSeqMiSeqDx in Research ModeNextSeq 550NovaSeq 6000
Compatitable Accessory Kit Nextera Index kit (24 hoặc 96 indexes)

 

Sản phẩm liên quan

DNA Sequencing Clean-Up

Reusable and simple purification kit for preparing sequence-ready DNA:
– Complete elimination of “Dye Blobs” for high quality Phred scores and long read lengths.
– Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps.
– Reusable!

DNA Clean & Concentrator (DCC)

Clean-up PCR and other enzymatic reactions in as little as 2 minutes:
– Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution in as little as 2 minutes with 0 μl wash residue carryover.
– Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
– Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

DNA Clean & Concentrator (DCC)

Clean-up PCR and other enzymatic reactions in as little as 2 minutes:
– Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution in as little as 2 minutes with 0 μl wash residue carryover.
– Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
– Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

Quick-DNA

Rapid purification of DNA from cells and whole blood:
– Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
– Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples. are fracture resistant and chemically insert.

GeneProof Hepatitis C Virus (HCV) PCR Kit

GeneProof Hepatitis C Virus (HCV) PCR Kit applies PCR technology to detect RNA of HCV virus in patient samples. The kit has CE-IVD certification for in vitro Diagnostic Use.

GeneProof Hepatitis B Virus (HBV) PCR Kit

GeneProof Hepatitis B Virus (HBV) PCR Kit applies PCR technology to detect DNA of HBV virus in patient samples. The kit has CE-IVD certification for in vitro Diagnostic Use.

TruSight Tumor 170 Kit

TruSight Tumor 170 Kit includes library preparation reagent set and uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 170 genes that are commonly related to mutations in solid tumors and somatic variants.

Công nghệ Microarray – ILMN Microarray Solutions

Công nghệ Microarray BeadArray được sử dụng trong các sản phẩm array của Illumina và các máy scanner trong một loạt các ứng dụng phân tích DNA và RNA.

Nextera DNA Flex Library Preparation Kit

Nextera DNA Flex Library Preparation là bộ kit được dùng để chuẩn bị thư viện DNA cho quá trình giải trình tự DNA trên hệ thống giải trình tự gen thế hệ mới (NGS – Next Generation Sequencing) của hãng Illumina

Nextera DNA Library Preparation Kit

Nextera DNA Library Preparation Kit là bộ kit chuẩn bị thư viện DNA cho quá trình giải trình tự DNA trên hệ thống giải trình tự gen thế hệ mới (NGS – Next Generation Sequencing) của hãng Illumina

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