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The Nextera DNA Flex Library Prep Kit provides library preparation reagent for DNA sequencing on Next Generation Sequencing system of Illumina.

Thông số kỹ thuật

Parameters
Assay Time ~ 3.5-4 hours
Hands-on Time 1-1.5 hours
Specialised Sample Types gDNA, Blood, Saliva, PCR Amplicons, Plasmids, Dried Blood Spots, Low Input Samples
DNA Input Type ·   Small Genomes: 1-500 ng DNA,

·   Large Genomes: 100-500 ng

Technology Human Whole Genome, Small Whole Genome, Large Whole Genome of any species including Human Genome
Samples/run Maximum 96 samples
Insert Size 300-350 bp
Variant Class Chromosomal Abnormalities, Copy Number Variants (CNVs), Gene Fusions, Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants, Structural Variants
NGS System Compatibility HiSeq 2500HiSeq 3000HiSeq 4000HiSeq X FiveHiSeq X TenMiniSeqMiSeqMiSeqDx in Research ModeNextSeq 550NovaSeq 6000
Compatitable Accessory Kit Nextera Index kit (24 hoặc 96 indexes)

 

Sản phẩm liên quan

Basic Yellow 40

Thông tin sản phẩm

Model: Basic Yellow

Hãng sản xuất: Lynn Peavey

Xuất xứ: Mỹ

Hệ thống tự động Biomek i5

Tối ưu hóa độ tin cậy và thời gian “walk-away”. Biomek i5 là hệ thống mới nhất trong các hệ thống xử lý chất lỏng và là hệ thống tốt nhất, dẫn đầu thị trường – được kết nối với các phần bổ sung theo đề xuất từ các khách hàng trên toàn cầu.

– Phù hợp với các quy trình công suất trung bình đến lớn

– 25 vị trí deck

– Khoảng thể tích pipet 0.5 µL-1,000 µL (Đa kênh) hoặc 0.5 µL-5,000 µL (Span-8)

– Đầu đa kênh đơn (96/384) hoặc pipet Span-8 có kẹp

Quick-RNA Viral

Viral DNA and RNA from all biological fluids:
– Quick recovery of viral RNA from plasma, serum and other samples.
– Omits the use of organic denaturants and proteases.
– High-quality viral RNA is ready for RT-PCR, sequencing, etc.

DNA Sequencing Clean-Up

Reusable and simple purification kit for preparing sequence-ready DNA:
– Complete elimination of “Dye Blobs” for high quality Phred scores and long read lengths.
– Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps.
– Reusable!

DNA Clean & Concentrator (DCC)

Clean-up PCR and other enzymatic reactions in as little as 2 minutes:
– Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution in as little as 2 minutes with 0 μl wash residue carryover.
– Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
– Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

Quick-DNA Fungal/Bacterial Kit

Easily isolate DNA from tough-to-lyse fungi and bacteria:
– Simple, efficient isolation of DNA from all types of tough-to-lyse fungi and bacteria in minutes.
– Ultra-high density BashingBeads are fracture resistant and chemically insert.

GeneProof Mycobacterium tuberculosis PCR Kit

The Health Statistics shows that there are 200,000 tuberculosis patients and 30,000 deaths every year in Vietnam. The main cause of human tuberculosis is M. Tuberculosis, also called as tuberculosis bacteria. 

GeneProof Mycobacterium tuberculosis PCR Kit applies PCR technology to detect DNA of Mycobacterium tuberculosis in samples. The kit has CE-IVD certification for in vitro Diagnostic Use.

GeneProof Hepatitis C Virus (HCV) PCR Kit

GeneProof Hepatitis C Virus (HCV) PCR Kit applies PCR technology to detect RNA of HCV virus in patient samples. The kit has CE-IVD certification for in vitro Diagnostic Use.

TruSight Tumor 170 Kit

TruSight Tumor 170 Kit includes library preparation reagent set and uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 170 genes that are commonly related to mutations in solid tumors and somatic variants.

Công nghệ Microarray – ILMN Microarray Solutions

Công nghệ Microarray BeadArray được sử dụng trong các sản phẩm array của Illumina và các máy scanner trong một loạt các ứng dụng phân tích DNA và RNA.

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