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Hệ thống giải trình tự thế hệ mới NextSeq 2000

Model: iScan

Manufacturer: Illumina / USA

Fully automated Micro-array System

Ứng dụng
Thông số kỹ thuật

 

– The iScan status indicator lights and scan bar on the front panel show the current status of the iScan: Power (blue) – Instrument is on; Ready (green check) – Instrument has been initialized and is ready to scan; Warning (amber triangle) – Instrument error has occurred

– The scanning system uses high-performance lasers, optics, and fully automated detection systems to provide extremely rapid scan times without sacrificing data quality, which helps to process complex data when running multiple samples

– With a high signal-to-noise ratio, high sensitivity, a low limit of detection, and a broad dynamic range, the iScan System can detect single copy number changes in gene expression and DNA methylation analysis

– The system uses microarray technology, the process of scanning and analyzing are carried out on the Beadchips.

– Beadchip is based on silicon particles with the size of 1-2 μm arranged in wells with a spacing of 5.7 μm between beads, each bead is covered by hundreds to thousands copies of specific oligonucleotides to generate a perfect hybridization environment in terms of large area, ensuring the highest hybridization yield.

– Ability to analyze high-quality data with high call rates (>99.9%), reducing the positive false and negative false.

– Normal Scan Function: manual loading

– Automatic Scan function: Automatic sample loading with robot

– Automatic Sample Running:

  • Automatic loading for 1 or 2 scanners
  • Samples can be run per week (hand loading): > 200 samples
  • Samples can be run per week (automatic loading): > 500 samples

– The Beadchip tray allows to analyze four Beadchips simultaneously

– The system operates simultaneously ≥24 samples per run on a Beadchip

– Number of samples / run varies by chemical reagent kits and applications

– Scan time for each sample varies by specialized kits and applications

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Biomek i7 Automated Workstation

Designed for your evolving workflow priorities Representing the best of what made Biomek an industry leading liquid handler—combined with enhancements suggested by customers around the globe—the Biomek i7 Automated Workstation has been designed to optimize workflows and increase walk-away in mid- to high-throughput labs.

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– Ideal for medium- to high-throughput workflows

– 25 deck positions

– 0.5 µL-1,000 µL (Multichannel) or 0.5 µL-5,000 µL (Span-8) pipetting volume ranges

– Single Multichannel head (96/384) or Span-8 pipetting with gripper

Quick-RNA Viral

Viral DNA and RNA from all biological fluids:
– Quick recovery of viral RNA from plasma, serum and other samples.
– Omits the use of organic denaturants and proteases.
– High-quality viral RNA is ready for RT-PCR, sequencing, etc.

DNA Sequencing Clean-Up

Reusable and simple purification kit for preparing sequence-ready DNA:
– Complete elimination of “Dye Blobs” for high quality Phred scores and long read lengths.
– Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps.
– Reusable!

DNA Clean & Concentrator (DCC)

Clean-up PCR and other enzymatic reactions in as little as 2 minutes:
– Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution in as little as 2 minutes with 0 μl wash residue carryover.
– Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
– Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

Quick-DNA

Rapid purification of DNA from cells and whole blood:
– Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
– Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.™ are fracture resistant and chemically insert.

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The Health Statistics shows that there are 200,000 tuberculosis patients and 30,000 deaths every year in Vietnam. The main cause of human tuberculosis is M. Tuberculosis, also called as tuberculosis bacteria. 

GeneProof Mycobacterium tuberculosis PCR Kit applies PCR technology to detect DNA of Mycobacterium tuberculosis in samples. The kit has CE-IVD certification for in vitro Diagnostic Use.

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Hệ thống giải trình tự thế hệ mới NextSeq 2000