Certificate

Brand

Thermo Fisher Scientific

Product Type

Instrument

Highlights

The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.

Application

Key Features:

• Efficient protocols minimize amplification time compared to previous-generation kits
• An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
• Enhanced intracolor balance simplifies interpretation of mixture samples
• The inclusion of 10 mini-STRs maximizes results from degraded samples
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility

Specifications

Product Line GlobalFiler™
Product Type PCR Amplification Kit
Shipping Condition Dry Ice
Starting Material DNA

Other information

The GlobalFiler kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, designed to deliver optimized results with even highly degraded samples.

Developed specifically for casework applications, the GlobalFiler kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The GlobalFiler kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM SElect and Identifiler primer sequences helps ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

Contents & Storage

Store at -15°C to -25°C upon receipt and 2 to 8°C after initial use for up to 6 months.

 

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This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
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• It provides Quantfiler™ kit results in less than 2 hours.

3500xL Genetic Analyzer for Human Identification

The 24-capillary 3500xL Genetic Analyzer sets the standard in capillary electrophoresis. The 3500-series instruments are the first genetic analyzers designed with a specific feature set and workflow for the Human Identification application. The complete product combines the instrument with Applied Biosystems™ reagents, consumables and software, as well as industry leading support, to provide an integrated HID system solution that significantly improves ease-of-use and application efficiencies.

• Increased Consistency – Reduced signal variation with factory standardization and optional in-lane normalization
• Real-Time Data Quality Assessment – On instrument data evaluation features provide rapid identification and re-injection of samples requiring additional processing
• Improved Thermal Control – Smaller oven design and improved door seal for more consistent data migration
• HID Specific Workflow – Pre-configured for Applied Biosystems™ amplification kits to simplify run setup and software navigation
• Effortless Consumable Installation – Convenient one-time use consumable packaging
• Consumable Tracking with RFID Technology – On-instrument, real-time monitoring of consumable consumption and expiration
• Enhanced Throughput – Faster data generation with 8 or 24 capillary arrays and shorter run times
• Improved Polymer Pump Design – Reduced waste and fewer bubbles for better data quality
• Simplified Instrument Installation & Operation – New solid-state laser eliminates need for high voltage wiring and external venting
• Streamlined Instrument Maintenance – HID specific performance check and maintenance scheduling tools
• Data Chain of Custody – Controlled access and tracking through Security, Audit and eSig features

SeqStudio™ 24 Flex Genetic Analyzer for Human Identification

Designed using capillary electrophoresis technology trusted by HID labs for over 25 years, the Applied Biosystems SeqStudio 24 Flex Genetic Analyzer for Human Identification with 24-capillary array delivers the flexibility and accuracy your work demands. Innovative capabilities, including a continuously accessible four-plate deck, can help you achieve reliable STR results efficiently. Features such as lab-friendly connectivity and easy, on-instrument training videos can help get you up and running quickly and minimize downtime.

Increased lab productivity
The SeqStudio 24 Flex Genetic Analyzer for HID is the genetic analyzer for HID fragment analysis and Sanger sequencing that delivers the high-quality data and reliable performance that forensic scientists expect, with design enhancements for improved flexibility, usability, connectivity, and serviceability.

Flexibility
• Accessibility—continuously load plates and reprioritize urgent samples without having to terminate the current run
• Increased walk-away capacity—four plate positions accommodating 8-tube strips and 96-well sample plates
• Work when and where you want—set up your plate and monitor run progress from anywhere using the Thermo Fisher Connect Platform.

Usability
• Designed for all levels of experience—simple one-button startup, autocalibration, and onscreen step by-step routine maintenance instructions and onboard self-help training videos
• Simplified capillary array installation—redesigned, more robust arrays with “click-and-slide” retractor tab and enhanced detection cell housing provide improved capillary protection, ease of handling, insertion, and positioning • Compatibility with Applied Biosystems 3500 Series genetic analyzer buffers and polymers
• Easy inventory management with radio-frequency identification (RFID)–enabled tracking of consumables usage
• No desktop computer required—integrated touchscreen computer, intuitive software, walk-through wizards, and Plate Manager 2.1 Software for simplified plate setup and instrument operation

Connectivity
Integrate the communication technologies and security options of today to propel laboratory efficiencies of tomorrow.
• Connect your way—integrate your instrument via local area network (LAN), Wi-Fi, USB ports, and LIMS compatibility
• Confidently walk away—monitor your run and data via the Connect Platform from your mobile phone, tablet, laptop, or remote desktop
• Data integrity and compliance—optional security, audit, and electronic signature (SAE) software for an electronic chain of custody

Serviceability
Applied Biosystems instrument innovation continues with additionally included digital service ecosystem for remote troubleshooting.*
• Reduced service visits—70% of issues remotely resolved
• Reduced instrument downtime—remotely resolve issues in minutes, not days, using onboard Smart Help and Remote Support features
• Real-time audio/video collaboration—the Remote Support feature brings our technicians into your laboratory using cutting-edge, security-minded digital tools
• Secure transfer of instrument performance data—send log and run files directly from the instrument with the push of a button using Smart Help

*Connectivity required for Smart Help and Remote Monitoring

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The 3500 analyzer is shipped with the following system components:

• Capillary Electrophoresis instrument.
• 3500 (8-capillary) array and POP™ polymer
• Human identification reagents and other consumables for system qualification.
• Dell™ computer workstation with flat-screen monitor.
• Integrated software for instrument control, data collection, quality control, and basecalling or sizing of samples.

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