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Hệ thống giải trình tự thế hệ mới NextSeq 2000

TruSight Tumor 15 Kit includes library preparation reagent set and uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 15 genes that are commonly related to mutations in solid tumors and somatic variants.

Ứng dụng
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Specifications:

Library Preparation Time Total 7 hours (3.5 hands-on hours)
Sequence Run Time 24 hours on MiniSeq or 27 hours on MiSeq
Technology Next-Generation Sequencing (NGS)
Input Quantity 20 ng DNA (10 ng × 2 reactions)
Gene Sequencing 2 × 151bp
TruSight Tumor 15 Gene List AKT1, GNA11, NRAS, BRAF, GNAQ, PDGFRA, EGFR, KIT, PIK3CA, ERBB2,  KRAS, RET, FOXL2, MET, TP53
Amplicon Size Average 150-175 bp
Specialised Sample Types FFPE, low input
NGS System Compatibility MiniSeq, MiSeq, MiSeqDx in Reseach Mode
Sample Output 8 samples/run when using MiniSeq High Throughput Kit, MiSeq v3 chemistry
Variant Frequency 5%
Amplicon Coverage 93.5% bases at ≥ 500×
Variant Class Insertions-Deletions (Indels), Somatic Variants

 

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Tách chiết DNA từ tế bào và máu toàn phần:
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Clean-up PCR and other enzymatic reactions in as little as 2 minutes:
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Hệ thống giải trình tự thế hệ mới NextSeq 2000