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TruSight Myeloid Sequencing Panel Kit includes reagent sets for preparing up to 1536 amplicons in 1 reaction and sequencing up to 96 libraries in 1 run on next-generation sequencing (NGS) system of Illumina

Thông số kỹ thuật

Specifications:

Assay Time 8 hours
Hands-on Time 3 hours
Technology Next-Generation Sequencing (NGS)
Input Quantity 50 ng
Amplicon 568 amplicons, amplicon size 250 bp
Targeted genes 54 genes frequently mutated in myeloid leukemia, target size 141 kb
Specialised Sample Types FFPE, low input
NGS System Compatibility MiniSeq, MiSeq, MiSeqDx in Reseach Mode, NextSeq 550
Sample Output 8 samples/run when using MiniSeq High Throughput Kit, MiSeq v3 chemistry
Exons covered > 95% at 500×
Target minimum coverage 500×
Variant Class  Insertions-Deletions (Indels), Somatic Variants

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