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Hệ thống giải trình tự thế hệ mới NextSeq 2000

Rapid purification of DNA from cells and whole blood:
– Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
– Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.™ are fracture resistant and chemically insert.

Ứng dụng
Thông số kỹ thuật

Elution Volume: ≥50 µl.
Size Range: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Equipment: Microcentrifuge, Vortex.
Purity: High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8.
Yield: Up to 25 µg total DNA is eluted into ≥50 µl (30 µl minimum) DNA Elution Buffer or water. Human whole blood yields 3-7 µg DNA per 100 µl of blood. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.
Sample Source: Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized.
Workflow: Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.

Thông tin chi tiết: https://www.zymoresearch.eu/quick-dna#kit_quick_dna=82&prep_size=18

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Quick-DNA

Tách chiết DNA từ tế bào và máu toàn phần:
– Dễ dàng tách chiết DNA từ máu toàn phần, buffy coat, mẫu tăm bông, hoặc mẫu nuôi cấy tế bào.
– Quy trình k cần sử dụng Proteinase K và các chất biến tính cho mẫu dịch sinh hoặc và mẫu nuôi cấy tế bào.

Quick-RNA Viral

Viral DNA and RNA from all biological fluids:
– Quick recovery of viral RNA from plasma, serum and other samples.
– Omits the use of organic denaturants and proteases.
– High-quality viral RNA is ready for RT-PCR, sequencing, etc.

DNA Sequencing Clean-Up

Reusable and simple purification kit for preparing sequence-ready DNA:
– Complete elimination of “Dye Blobs” for high quality Phred scores and long read lengths.
– Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps.
– Reusable!

DNA Clean & Concentrator (DCC)

Clean-up PCR and other enzymatic reactions in as little as 2 minutes:
– Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution in as little as 2 minutes with 0 μl wash residue carryover.
– Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
– Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

Quick-DNA Fungal/Bacterial Kit

Easily isolate DNA from tough-to-lyse fungi and bacteria:
– Simple, efficient isolation of DNA from all types of tough-to-lyse fungi and bacteria in minutes.
– Ultra-high density BashingBeads™ are fracture resistant and chemically insert.

GeneProof Hepatitis B Virus (HBV) PCR Kit

GeneProof Hepatitis B Virus (HBV) PCR Kit applies PCR technology to detect DNA of HBV virus in patient samples. The kit has CE-IVD certification for in vitro Diagnostic Use.

Microarray System for Cancer Research and Cell Genetics

Model: iScan

Manufacturer: Illumina / USA

Fully automated Micro-array System

TruSight Exome Library Prep Kit

TruSight Exome Library Preparation Kit bao gồm bộ hóa chất để chuẩn bị thư viện kết hợp làm giàu đoạn exome để giải trình tự trên hệ thống giải trình tự gen thế hệ mới (NGS – Next Generation Sequencing) của hãng Illumina

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Hệ thống giải trình tự thế hệ mới NextSeq 2000