Certificate

Brand

illumina

Product Type

Instrument

Highlights

- Data passing through the filter: the number of clusters passing through the filter ranges from 8-25 million - Capacity per run: ranges from 2.5 GB - 7.5 GB data output - Data Quality (Quality Score) > Q30 (accuracy 99.9%)

Model: MiniSeq
Manufacturer: Illumina/USA
– New generation DNA sequencing system using synthetic sequencing technology: Sequencing by Synthesis – SBS
– Next Generation Sequencing, allowing to run the entire DNA and RNA amplification process combined with sequencing analysis on a single system
– Maximum reading capacity: 7.5 Gb (equivalent to 7.5 billion bases) within 24 hours
– Data Quality (Quality Score) > Q30 (accuracy 99.9%)

Application

– Whole-genome sequencing for small genomes (Small Whole-genome Resequencing)

– Targeted sequencing

– Sequencing to detect gene mutations related to colorectal cancer, lung cancer, … application in early screening and targeted treatment

– Sequencing mRNA, small RNA, micro RNA

– Paired-end sequencing of target amplicons

– Paired-end gene sequencing for target gene expression profiling

Specifications

– Sequencing time when using the High-Output Reagent Kit
• Sequencing 1 x 50 bp in 7 hours (384 samples)
• Sequencing 2 x 75 bp in 13 h (23 samples)
• 24h 2 x 150 bp sequencing (50 small genome sequencing samples, 28 target amplicon sequencing samples)
Compatible with 2 types of flow cells with different capacities (large capacity and medium capacity), depending on the purpose of sequencing
– Data passing through the filter: the number of clusters passing through the filter ranges from 8-25 million
– Capacity per run: ranges from 2.5 GB – 7.5 GB data output
– Data Quality (Quality Score) > Q30 (accuracy 99.9%). Percentage of data with quality > 85% > Q30 (1-way solves 25 million readings, 2-way gets 50 million reads)
– Optical system using excitation LEDs (515 nm, 650 nm)
Fully automated process: 10 min of manual operation and 6 h of sequencing (1 x 36 bp) including clustering, sequencing, and automatic washing after run
– Has radio wave consumables (RFID – tracking) feature, using pre-loaded chemical cartridges
Running capacity: up to 384 samples can be analyzed in one sequencing

Other information

Supplier: Illumina

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