Thermo Fisher Scientific
Instrument
The Applied Biosystems HID NIMBUS Presto System is an easy-to-use, compact instrument that combines the KingFisher Presto Purification System with an industry leading automated liquid handling workstation to precisely purify nucleic acids from lysed samples for use in sensitive downstream analyses such as DNA quantification and STR amplification.
| Sample Type | Trace |
| Description | Input: 1.5 mL Microcentrifuge tube, Spin and Filter 96-well plate, KingFisher 96-well Deep well plate Output: 1.5 mL Microcentrifuge tube, KingFisher 96-well Deep well plate, MicroAmp PCR 96-well plate |
| For Use With (Application) | Purification of Nucleic Acid from lysed samples |
| High-throughput Compatibility | 1 to 96 samples |
| Run Time | Can process 96 samples in <90 min. |
| Sample Volume | Please refer to the PrepFiler and PrepFiler BTA User Bulletin |
| Operating Environment | 0°C to 40°C (32°F to 104°F) |
| Depth (English) | 53.5 in |
| Depth (Metric) | 135.9 cm |
| Height (English) | 35 in. (door closed), 51.2 in. (door opened) |
| Height (Metric) | 88.9 cm (door closed), 130 cm (door opened) |
| Weight (English) | 247 lb. (Nimbus), 52.9 lb. (Presto) |
| Weight (Metric) | 112.1 kg (Nimbus), 24 kg (Presto) |
| Width (English) | 27.9 in. |
| Width (Metric) | 70.9 cm |
| Electrical Requirements | Power input: 100/240 VAC, 50 to 60 Hz, 5 A Output Power: 42 VDC, ±5%; 600 W max. |
| Frequency | 50/60 Hz |
Features of the HID NIMBUS Presto System include:
• Integrated magnetic particle-based technology for DNA purification in an automated liquid handling platform
• Purify more samples with less hands-on time—up to 96 samples in less than 90 minutes
• Optimized and validated scripts for use with PrepFiler and PrepFiler BTA automated forensic DNA extraction kits
• Uses various consumable plasticware such as 1.5-mL microfuge tubes, 96-well PCR plates, and 96-deep-well plates
• Easy-to-use software interface; start your method in just a few clicks
• Deck layout designed to accommodate further downstream workflows (e.g., quantitation and amplification)
The HID NIMBUS Presto System is optimized and validated for use with PrepFiler and PrepFiler BTA automated forensic DNA extraction kits according to the Scientific Working Group for DNA Analysis Methods (SWGDAM, December 2016).


The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.


The HID VeritiPro Thermal Cycler features a 96-well (0.2 mL) format with six independent temperature zones to provide precise temperature control and rapid temperature changes for conducting polymerase chain reactions (PCR). The HID VeritiPro Thermal Cycler features a powerful color touch screen and a USB port to simplify instrument setup and use.


The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.
This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.


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The SL-450 is ruggedly portable and cable-free while allowing users to move unhindered around any crime scene location. Its powerful 35W Xenon lamp is powered by a lithium-ion battery that provides 90 minutes of continuous power.


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