Certificate

Brand

Thermo Fisher Scientific

Product Type

Instrument

Highlights

The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.

Application

Key Features:

• Efficient protocols minimize amplification time compared to previous-generation kits
• An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
• Enhanced intracolor balance simplifies interpretation of mixture samples
• The inclusion of 10 mini-STRs maximizes results from degraded samples
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility

Specifications

Product Line GlobalFiler™
Product Type PCR Amplification Kit
Shipping Condition Dry Ice
Starting Material DNA

Other information

The GlobalFiler kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, designed to deliver optimized results with even highly degraded samples.

Developed specifically for casework applications, the GlobalFiler kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The GlobalFiler kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM SElect and Identifiler primer sequences helps ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

Contents & Storage

Store at -15°C to -25°C upon receipt and 2 to 8°C after initial use for up to 6 months.

 

Related Products

GlobalFiler™ Express PCR Amplification Kit and Prep-n-Go™ Buffer (for buccal swabs)

The GlobalFiler Express PCR Amplification Kit and Prep-n-Go Buffer (for buccal swabs) combines the features of our GlobalFiler Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer (for buccal swabs). The GlobalFiler Express kit is the first 6-dye, 24-locus STR kit that enables direct PCR amplification, eliminating the need to perform DNA extraction or purification steps for DNA database and other single-source reference samples. Prep-n-Go Buffer (for buccal swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

AutoMate Express™ Forensic DNA Extraction System, with service install

The AutoMate Express Forensic DNA Extraction System is an easy to use, robust bench-top instrument that utilizes the PrepFiler Express and PrepFiler Express BTA chemistries packaged in pre-filled, foil sealed cartridges. We have designed the PrepFiler chemistry specifically to improve the quantity and quality of DNA isolated from forensic samples, thereby increasing the potential to obtain maximum information from downstream STR analysis.

QuantStudio™ 5 Real-Time PCR System for Human Identification, 96-well, 0.2 mL, desktop

The Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification with tower computer is the latest addition to our human identification (HID) real-time PCR family of products. This instrument is designed for both new and experienced users who need a simple, reliable, and affordable real-time PCR system that doesn’t require compromise on performance or quality. When used with our latest advances in quantification chemistry and HID Real-Time PCR Analysis Software, this system offers a sensitive, robust solution for forensic DNA quantification. It also helps prepare laboratories for future quantification technologies. With maximum dye versatility, this HID solution offers accurate, trusted results in a small benchtop footprint.

Contents & Storage

Desktop Computer

7500 Real-Time PCR System for Human Identification with Dell Notebook D520

The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.

This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.

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