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- 7500 Real-Time PCR System for Human Identification with Dell Notebook D520
Certificate
Brand
Thermo Fisher Scientific
Product Type
Instrument
Highlights
For Research Use Only
The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.
This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.
Specifications
| Format | 96-well Plate |
| Thermal Accuracy | 0.25°C (35°C to 95°C after 3 min.) |
| Sample Ramp Rate | ± 1.1°C⁄s |
| Sensitivity | 1 Copy of RNase P gene (Human Genomic DNA) |
| Temperature Range (Metric) | 4 to 100°C |
| Reaction Volume Range | 20-100 µl |
| Passive Reference Dye | ROX (Separate Tube), No ROX, ROX (Pre-mixed) |
| Dynamic Range | 9 Logs |
| Calibrated Dye | SYBR™ Green I, ROX™, Texas Red™, NED™, JOE™, Cy3™, Cy5™, VIC™, FAM™, TAMRA™ |
| High-throughput Compatibility | Multiplexing |
| For Use With (Equipment) | 7500 System |
| Thermal Uniformity | 0.50°C (after 30 s) |
| Reaction Speed | Standard |
| Throughput | 4 to 5-96-well Plates per 8-hr day |
| Optics | CCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters |
| Thermal Cycling System | Peltier-based System |
| Dimensions | Width 34 cm (13.39 in.), Depth 45 cm (17.72 in.), Height 49 cm (19.29 in.) |
| External Computer | Dell Notebook D520 |
| Software Compatibility | GeneMapper™ ID v3.2, 7500 System Software, HID Real Time PCR Analysis Software v1.1 |
| Precision | 99.7% Confidence (5000 & 10000 Copies) |
| Voltage | 120 V |
| Electrical Requirements | 950 W |
| Peak Block Ramp Rate | 2.5°C⁄s |
| Weight | 75 lb. |
Other information
Built for Performance
The 7500 system for Human Identification includes SDS v.1.2.3 software which is validated to run Quantifiler™ Human DNA and Quantifiler™ Y Human Male DNA quantification kits. SDS v1.2.3 is a powerful, versatile software that includes plate set-up wizards and automated analysis tools that make data processing easier. Its advanced data viewing capabilities make analysis less time consuming. The instrument is a powerful, five-color platform that is calibrated for the broadest range of dyes available: FAM™⁄SYBR™ Green I, VIC™⁄JOE™, TAMRA™⁄CY3, ROX™⁄Texas Red, and CY5 dyes. Its specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets and its advanced multi-componenting algorithm minimizes spectral crosstalk making the instrument superior for multiplexing.
Complete Validated Solution
Applied Biosystems™ offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR™ STR kits, instrumentation, software, and consumables. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD. The system has been internally validated by Applied Biosystems™ following DAB⁄SWGDAM guidelines for use with Quantifiler™ Human Quantification System and Quantifiler™ Y Human Male DNA Quantification System making the 7500 system suitable for casework, paternity, and databasing laboratories.
EXPERIENCE MATTERS™
When you partner with Applied Biosystems™ you gain access to the extensive knowledge and experience of our forensic support team and training resources dedicated to Human Identification laboratories. Our HID University™ provides continuing education for DNA analysts, our support and Applications Scientists offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.
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Package Components
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A36187
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A36187
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A46208
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A46208
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