Certificate

Brand

Thermo Fisher Scientific

Product Type

Instrument

Highlights

For Research Use Only

The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.

This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.

Specifications

Format 96-well Plate
Thermal Accuracy 0.25°C (35°C to 95°C after 3 min.)
Sample Ramp Rate ± 1.1°C⁄s
Sensitivity 1 Copy of RNase P gene (Human Genomic DNA)
Temperature Range (Metric) 4 to 100°C
Reaction Volume Range 20-100 µl
Passive Reference Dye ROX (Separate Tube), No ROX, ROX (Pre-mixed)
Dynamic Range 9 Logs
Calibrated Dye SYBR™ Green I, ROX™, Texas Red™, NED™, JOE™, Cy3™, Cy5™, VIC™, FAM™, TAMRA™
High-throughput Compatibility Multiplexing
For Use With (Equipment) 7500 System
Thermal Uniformity 0.50°C (after 30 s)
Reaction Speed Standard
Throughput 4 to 5-96-well Plates per 8-hr day
Optics CCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters
Thermal Cycling System Peltier-based System
Dimensions Width 34 cm (13.39 in.), Depth 45 cm (17.72 in.), Height 49 cm (19.29 in.)
External Computer Dell Notebook D520
Software Compatibility GeneMapper™ ID v3.2, 7500 System Software, HID Real Time PCR Analysis Software v1.1
Precision 99.7% Confidence (5000 & 10000 Copies)
Voltage 120 V
Electrical Requirements 950 W
Peak Block Ramp Rate 2.5°C⁄s
Weight 75 lb.

Other information

Built for Performance
The 7500 system for Human Identification includes SDS v.1.2.3 software which is validated to run Quantifiler™ Human DNA and Quantifiler™ Y Human Male DNA quantification kits. SDS v1.2.3 is a powerful, versatile software that includes plate set-up wizards and automated analysis tools that make data processing easier. Its advanced data viewing capabilities make analysis less time consuming. The instrument is a powerful, five-color platform that is calibrated for the broadest range of dyes available: FAM™⁄SYBR™ Green I, VIC™⁄JOE™, TAMRA™⁄CY3, ROX™⁄Texas Red, and CY5 dyes. Its specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets and its advanced multi-componenting algorithm minimizes spectral crosstalk making the instrument superior for multiplexing.

Complete Validated Solution
Applied Biosystems™ offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR™ STR kits, instrumentation, software, and consumables. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD. The system has been internally validated by Applied Biosystems™ following DAB⁄SWGDAM guidelines for use with Quantifiler™ Human Quantification System and Quantifiler™ Y Human Male DNA Quantification System making the 7500 system suitable for casework, paternity, and databasing laboratories.

EXPERIENCE MATTERS™
When you partner with Applied Biosystems™ you gain access to the extensive knowledge and experience of our forensic support team and training resources dedicated to Human Identification laboratories. Our HID University™ provides continuing education for DNA analysts, our support and Applications Scientists offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.

Related Products

GlobalFiler™ Express PCR Amplification Kit and Prep-n-Go™ Buffer (for buccal swabs)

The GlobalFiler Express PCR Amplification Kit and Prep-n-Go Buffer (for buccal swabs) combines the features of our GlobalFiler Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer (for buccal swabs). The GlobalFiler Express kit is the first 6-dye, 24-locus STR kit that enables direct PCR amplification, eliminating the need to perform DNA extraction or purification steps for DNA database and other single-source reference samples. Prep-n-Go Buffer (for buccal swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

GlobalFiler™ PCR Amplification Kit

The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.

HID Nimbus™ Presto System

The Applied Biosystems HID NIMBUS Presto System is an easy-to-use, compact instrument that combines the KingFisher Presto Purification System with an industry leading automated liquid handling workstation to precisely purify nucleic acids from lysed samples for use in sensitive downstream analyses such as DNA quantification and STR amplification.

ProFlex™ 96-well PCR System

The ProFlex 96-well PCR system is the latest in our line of Applied Biosystems thermal cyclers. The ProFlex system combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.

The ProFlex 96-well PCR system allows you to:
• Interchange the 96-well 0.2-mL block with another block type if throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate an old instrument with Thermal Simulation modes
• Optimize PCR quickly and efficiently with better-than-gradient VeriFlex blocks

HID VeritiPro™ Thermal Cycler, 96 well, 0.2 mL

The HID VeritiPro Thermal Cycler features a 96-well (0.2 mL) format with six independent temperature zones to provide precise temperature control and rapid temperature changes for conducting polymerase chain reactions (PCR). The HID VeritiPro Thermal Cycler features a powerful color touch screen and a USB port to simplify instrument setup and use.

3500 Genetic Analyzer for Human Identification

The 3500 analyzer is shipped with the following system components:

• Capillary Electrophoresis instrument.
• 3500 (8-capillary) array and POP™ polymer
• Human identification reagents and other consumables for system qualification.
• Dell™ computer workstation with flat-screen monitor.
• Integrated software for instrument control, data collection, quality control, and basecalling or sizing of samples.

BattleLite Forensic Blue Light Flashlight Kit

Product Information

Model: 56031

Manufacturer: Lynn Peavey

Origin: United States

Fold-A-Lab kit-portable sauna cabinet

Product Information

Model: Original Fold-A-Lab Kit

Manufacturer: Lynn Peavey

Origin: United States

Fuming Hot Box

Product Information

Model: Fuming Hot Box

Manufacturer: Lynn Peavey

Origin: United States

Basic Yellow 40

Product Information

Model: Basic Yellow

Manufacturer: Lynn Peavey

Origin: United States