Thermo Fisher Scientific
Instrument
For Research Use Only
The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.
This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.
Format | 96-well Plate |
Thermal Accuracy | 0.25°C (35°C to 95°C after 3 min.) |
Sample Ramp Rate | ± 1.1°C⁄s |
Sensitivity | 1 Copy of RNase P gene (Human Genomic DNA) |
Temperature Range (Metric) | 4 to 100°C |
Reaction Volume Range | 20-100 µl |
Passive Reference Dye | ROX (Separate Tube), No ROX, ROX (Pre-mixed) |
Dynamic Range | 9 Logs |
Calibrated Dye | SYBR™ Green I, ROX™, Texas Red™, NED™, JOE™, Cy3™, Cy5™, VIC™, FAM™, TAMRA™ |
High-throughput Compatibility | Multiplexing |
For Use With (Equipment) | 7500 System |
Thermal Uniformity | 0.50°C (after 30 s) |
Reaction Speed | Standard |
Throughput | 4 to 5-96-well Plates per 8-hr day |
Optics | CCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters |
Thermal Cycling System | Peltier-based System |
Dimensions | Width 34 cm (13.39 in.), Depth 45 cm (17.72 in.), Height 49 cm (19.29 in.) |
External Computer | Dell Notebook D520 |
Software Compatibility | GeneMapper™ ID v3.2, 7500 System Software, HID Real Time PCR Analysis Software v1.1 |
Precision | 99.7% Confidence (5000 & 10000 Copies) |
Voltage | 120 V |
Electrical Requirements | 950 W |
Peak Block Ramp Rate | 2.5°C⁄s |
Weight | 75 lb. |
Built for Performance
The 7500 system for Human Identification includes SDS v.1.2.3 software which is validated to run Quantifiler™ Human DNA and Quantifiler™ Y Human Male DNA quantification kits. SDS v1.2.3 is a powerful, versatile software that includes plate set-up wizards and automated analysis tools that make data processing easier. Its advanced data viewing capabilities make analysis less time consuming. The instrument is a powerful, five-color platform that is calibrated for the broadest range of dyes available: FAM™⁄SYBR™ Green I, VIC™⁄JOE™, TAMRA™⁄CY3, ROX™⁄Texas Red, and CY5 dyes. Its specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets and its advanced multi-componenting algorithm minimizes spectral crosstalk making the instrument superior for multiplexing.
Complete Validated Solution
Applied Biosystems™ offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR™ STR kits, instrumentation, software, and consumables. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD. The system has been internally validated by Applied Biosystems™ following DAB⁄SWGDAM guidelines for use with Quantifiler™ Human Quantification System and Quantifiler™ Y Human Male DNA Quantification System making the 7500 system suitable for casework, paternity, and databasing laboratories.
EXPERIENCE MATTERS™
When you partner with Applied Biosystems™ you gain access to the extensive knowledge and experience of our forensic support team and training resources dedicated to Human Identification laboratories. Our HID University™ provides continuing education for DNA analysts, our support and Applications Scientists offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.
The GlobalFiler Express PCR Amplification Kit and Prep-n-Go Buffer (for buccal swabs) combines the features of our GlobalFiler Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer (for buccal swabs). The GlobalFiler Express kit is the first 6-dye, 24-locus STR kit that enables direct PCR amplification, eliminating the need to perform DNA extraction or purification steps for DNA database and other single-source reference samples. Prep-n-Go Buffer (for buccal swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.
The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.
The Applied Biosystems HID NIMBUS Presto System is an easy-to-use, compact instrument that combines the KingFisher Presto Purification System with an industry leading automated liquid handling workstation to precisely purify nucleic acids from lysed samples for use in sensitive downstream analyses such as DNA quantification and STR amplification.
The Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification with tower computer is the latest addition to our human identification (HID) real-time PCR family of products. This instrument is designed for both new and experienced users who need a simple, reliable, and affordable real-time PCR system that doesn’t require compromise on performance or quality. When used with our latest advances in quantification chemistry and HID Real-Time PCR Analysis Software, this system offers a sensitive, robust solution for forensic DNA quantification. It also helps prepare laboratories for future quantification technologies. With maximum dye versatility, this HID solution offers accurate, trusted results in a small benchtop footprint.
The Applied Biosystems SeqStudio Genetic Analyzer for HID is a low-throughput, easy-to-use, convenient benchtop system that delivers gold-standard fragment analysis or Sanger sequencing with just a simple click. It is validated across a broad range of HID forensic kits to deliver answers you can trust. The SeqStudio Genetic Analyzer couples the latest advancements in touchscreen usability with the same data quality, reliability, and support you have come to expect from Applied Biosystems instruments.
A36187
Catalog Number:
Unit Size:
Qty:
|
A36187
1 |
A46208
Catalog Number:
Unit Size:
Qty:
|
A46208
1 |
The 3500 analyzer is shipped with the following system components:
• Capillary Electrophoresis instrument.
• 3500 (8-capillary) array and POP™ polymer
• Human identification reagents and other consumables for system qualification.
• Dell™ computer workstation with flat-screen monitor.
• Integrated software for instrument control, data collection, quality control, and basecalling or sizing of samples.
Amped Authenticate is a software application for forensic image authentication, tampering detection, and camera ballistics on digital images. This product is the only image authentication software on the market that offers a complete suite of powerful tools to exploit the data behind digital images, allowing for the analysis of image integrity, authenticity, metadata, source and history, and detection of tampering prior to its use as intelligence and evidence. With a single product, several tests can be performed to determine if an image has been manipulated as well as verify if a photo was taken from a specific device.
SUITCASE SCENE EXAMINATION VERSATILE
Model: 56031
Manufacturer: Lynn Peavey
Origin: United States
Model: Army Compact Kit
Manufacturer: Lynn Peavey
Origin: United States