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Next-Generation Sequencing System – NextSeq 2000

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Instrument

Highlights

The Applied Biosystems™ 3500 Series Genetic Analyzers are specifically designed to support the demanding performance needs of validated and regulated environments while retaining the unsurpassed application versatility that life science researchers expect. The 24-capillary 3500xL Genetic Analyzers continue to set the standard in capillary electrophoresis.

Application
Reliability
• High accuracy—using BigDye™ Terminator v1.1 or v3.1 Sequencing Standards, the instrument has shown >99% read accuracy
• Proven results—Applied Biosystems genetic analyzers are a trusted standard for both Sanger sequencing and fragment analysis
Flexibility
• Provides a dual mode user interface: the Diagnostic Mode and the Research Use Only Mode
• Enables both fragment analysis and Sanger sequencing in the secure Diagnostic Mode
• Enables import of validated sequencing and fragment analysis assay protocols to run in Diagnostic Mode
• Offers different configurations to meet your throughput needs: this 24-capillary version and an 8-capillary version
Simplicity and efficiency
• Reduced instrument hands-on time with simplified installation
• Controlled and efficient run setup with intuitive user interface, preconfigured plate templates, and software-controlled functions (plate setup, data collection, analysis)
• Maintenance calendar with programmable prompts and service history functionality
• RFID (Radio Frequency Identification) tracking of consumables, built-in quality controls, and real-time data quality evaluation
• SAE (Security, Audit, and Electronic) signature capability to assist with compliance to governing regulations
Specifications

– Number of capillaries: 24 capillaries
– Compatible capillary length: 36 cm or 50 cm
– Number of fluorescent dyes: 6 colors
– Capacity: 2 plates, compatible with 96-well plates, 384-well plates or 8-tube strips
– Compatible with dye sets: E, Z, F, E5, D, G5, J6, J6-T
– Polymer: POP-4™, POP-6™, POP-7™
‘- Sample throughput: ≥1104 samples/day for sequencing, ≥1272 for fragment analysis
‘- Average read length in 90% of samples ≥ 850 bases
– Minimum run time: ≤30 minutes

Other information

– Dimensions: 61 cm x 61 cm (122 cm open) x 72 cm (LxWxH)
– Weight: 82 kg
– Operating conditions:
+ Temperature 15°C–30°C
+ Humidity: 20-80%
+ Power supply: 100–240V (±10%); 50–60 Hz (±10%)
+ Electrophoresis voltage: Up to 20 kV

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Products of interest

Batch Convert Unplayable Videos

Zymot ICSI

Next-Generation Sequencing System – NextSeq 2000