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Hệ thống giải trình tự thế hệ mới NextSeq 2000

Rapid purification of DNA from cells and whole blood:
– Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
– Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.™ are fracture resistant and chemically insert.

Ứng dụng
Thông số kỹ thuật

Elution Volume: ≥50 µl.
Size Range: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Equipment: Microcentrifuge, Vortex.
Purity: High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8.
Yield: Up to 25 µg total DNA is eluted into ≥50 µl (30 µl minimum) DNA Elution Buffer or water. Human whole blood yields 3-7 µg DNA per 100 µl of blood. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.
Sample Source: Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized.
Workflow: Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.

Thông tin chi tiết: https://www.zymoresearch.eu/quick-dna#kit_quick_dna=82&prep_size=18

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Hệ thống giải trình tự thế hệ mới NextSeq 2000