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Hệ thống giải trình tự thế hệ mới NextSeq 2000

BeadArray microarray technology is deployed on either of two multi-sample array formats for DNA or RNA analysis applications.

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Thông số kỹ thuật

– BeadArray microarray technology is based on 3-micron silica beads that self assemble in microwells on either of two substrates: fiber optic bundles or planar silica slides. When randomly assembled on one of these two substrates, the beads have a uniform spacing of ~5.7 microns. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide that act as the capture sequences in a given Illumina assay.

– With multi-sample BeadChip formats, uniform pits are etched into the surface of each substrate to a depth of approximately 3 microns prior to assembly. Beads are then randomly assembled and held in these microwells by Van der Waals forces and hydrostatic interactions with the walls of the well.

– BeadArray product density advancements have increased genomic coverage for whole-genome genotyping, resolution for cytogenetics and CNV detection, and sample throughput for DNA methylation and focused genotyping.

– Illumina microarrays offer several advantages:
+ Trusted data quality
+ Widespread global adoption, collaboration fostering and accelerating research acceleration
+ Detection of chromosomal alterations and copy number variation (CNV)
+ Scalable workflow for large studies, including population-scale genotyping
+ Integration with Illumina sequencing systems for multi-omics analyses
+ Flexible content
+ Built-in laboratory management, tracking, and traceability tools

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Hệ thống giải trình tự thế hệ mới NextSeq 2000