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Next-Generation Sequencing System – NextSeq 2000

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TruSight Tumor 26 provides amplicon-based library preparation reagents, DNA QC, sample indexes, and oligos targeting identified regions of interest.

TruSight Tumor 26 Kit includes library preparation reagent set and uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 26 genes that are commonly related to mutations in solid tumors and somatic variants

Application

TruSight® Tumor 26 takes a deeper view of variation in solid tumors including lung, colon,
melanoma, gastric, and ovarian. This step enables clinical researchers to look beyond point
mutations within hot spots in single genes for a more comprehensive view of somatic
variation. TruSight Tumor 26 provides amplicon-based library preparation reagents, DNA
QC, sample indexes, and oligos targeting identified regions of interest. Sufficient reagents
are supplied for 48 samples and the indexes provided enable indexing of 4 samples per sequencing run

Specifications

Specifications:

Library Preparation Time Total <7 hours (2.5 hands-on hours)
Sequence Run Time 22 hours MiSeq, up to 33 hours on HiSeq 2500
Technology Next-Generation Sequencing (NGS)
Input Quantity Based on QC samples, typically in 30-300 ng DNA
Gene Sequencing 2 × 121bp
TruSight Tumor 26 Gene List AKT1, ALK, APC, NRAS, BRAF, CDH1, CTNNB1, EGFR, ERBB2, FBXW7, FGFR2, FOXL2, GNAQ, GNAS, KIT, KRAS, MAP2K1, MET, MSH6, NRAS, PDGFRA, PIK3CA, PTEN, SMAD4, SRC, STK11, TP53
Amplicon Size 174 amplicons, average size 165-195 bp
Specialised Sample Types FFPE
NGS System Compatibility MiniSeq, MiSeq, MiSeqDx ở chế độ Reseach Mode, NextSeq 550
Sample Output 4 samples/run on MiSeq

48 samples when running on HiSeq 2500 in Rapid run mode
Limit of Detection 3%
Variant Frequency ≤5%
Amplicon Coverage Minimum 1000×, average7000×
Variant Class Insertions-Deletions (Indels), Somatic Variants

 

Other information

Supplier

Illumina

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Next-Generation Sequencing System – NextSeq 2000