TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of soma in NGS – Next Generation Sequencing of Illumina
illumina
Reagents
26 Variants detection from Somatic cells
TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of soma in NGS – Next Generation Sequencing of Illumina
TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of soma in NGS – Next Generation Sequencing of Illumina
List | Parameter |
Time preparation | Total <7h (including, 2.5h to manual) |
Sequencing time | 22h in MiSeq system, upto 33h in HiSeq 2500 system |
Technology | Next Generation Sequencing |
Input sample | Based on QC, typically appx. 30-300 ng DNA |
Sequencing | 2 × 121bp |
15 gene được giải trình tự | AKT1, ALK, APC, NRAS, BRAF, CDH1, CTNNB1, EGFR,
ERBB2, FBXW7, FGFR2, FOXL2, GNAQ, GNAS, KIT, KRAS, MAP2K1, MET, MSH6, NRAS, PDGFRA, PIK3CA, PTEN, SMAD4, SRC, STK11, TP53 |
Amplicon | Include 174 amplicon with average size 165-195 bp |
Sample | FFPE |
Compatible NGS system | MiniSeq, MiSeq, MiSeqDx ở chế độ Reseach Mode, NextSeq 550 |
Sample running capacity | 4 sample/time on MiSeq48 sample running on HiSeq 2500 (rapid-run mode) |
Limit detection | 3% |
Frequency | ≤5% |
Coverage | Min 1000×, avg.7000× |
Variants detection | Indels, changing of genetics in Somatic cells |
Supplier
Illumina
BRCAaccuTest™ PLUS with CE-IVD certificate for HBOC Breast/Ovarian Cancer Test
Model: NovaSeq 6000
Manufacturer: Illumina/USA
– Scalable platform
Match data output, time to results, and price per sample to study needs
– Flexible performance
Configure sequencing method, flow cell type, and read length to support a broad range of applications
– Streamlined operation
Increase lab efficiency with a simplified workflow and reduced hands-on time
The NextSeq 2000 Sequencing System uses patterned flow cells similar to those that power the NovaSeq™ 6000 System. The result is a highly flexible and scalable benchtop system that offers the highest cluster density flow cell of any on-market NGS system to date, driving down the cost per gigabase (Gb) of the sequencing run.
The PANA RealTyper™ HPV Screening Kit is a CE marked diagnostic device in accordance with the European Union in vitro Diagnostic Medical Device Directive 98/79/EC
DxFLEX is a new clinical flow cytometry platform derived from the successful CytoFLEX. The advanced sensitivity and intuitive software DxFLEX makes flow cytometry routine for both novice and expert flow cytometry technicians and promotes standardization. Functionality in the autoloader facilitates accurate results and sample tracking.
Model: NovaSeq 6000
Manufacturer: Illumina/USA
– Scalable platform
Match data output, time to results, and price per sample to study needs
– Flexible performance
Configure sequencing method, flow cell type, and read length to support a broad range of applications
– Streamlined operation
Increase lab efficiency with a simplified workflow and reduced hands-on time
Model: iSeq 100
Manufacturer: Illumina/USA
Illumina’s smallest next-generation DNA sequencing system, with small, fast and efficient sequencing throughput, suitable for all labs
– Fast data generation: Suitable for small projects, on a dedicated device, low throughput with fast turnaround times
– Convenient operation: Control the sequencing process from start to finish and ensure independent sequencing instead of outsourcing.
TruSight Tumor 26 Kit includes library preparation reagent set and uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 26 genes that are commonly related to mutations in solid tumors and somatic variants
Developed with cancer genomics experts in collaboration, oligos ready-to-use, pre-engineered oligos allow researchers to sequence a wide range of genes and single nucleotide polymorphisms (SNPs) in advance. this is related to cancer predisposition
Model: Nextseq 550
Manufacturer: Illumina/USA
– Is the only sequencing system capable of deploying applications running sequencing and reading microarray biochips
– New generation DNA sequencing system using synthetic sequencing technology: Sequencing by Synthesis – SBS
– The whole process of gene sequencing is conducted on the surface of flow cells, convenient to use and easy to manipulate.