Instrument
The GlobalFiler Express PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler Express kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler PCR Amplification Kit.
Product Line | GlobalFiler™ |
Product Type | Express PCR Amplification Kit |
Shipping Condition | Dry Ice |
Key Features:
• Up to 5-times faster amplification time than previous-generation kits
• DNA results in <2 hours—using current CE platforms
• Optimized protocols for blood and buccal samples deposited on swab or paper substrates
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility
The GlobalFiler Express kit is a robust and reliable single-amplification STR multiplex kit optimized for use with database and single-source samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases and utilizes optimized reagents, thermal cycling parameters, and the latest advances in oligo synthesis and purification to enable the highest quality results specific to the sample type being processed. Maintenance of the NGM SElect and Identifiler primer sequences helps to ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).
Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.
The Applied Biosystems™ 3500 Series Genetic Analyzers are specifically designed to support the demanding performance needs of validated and regulated environments while retaining the unsurpassed application versatility that life science researchers expect. The 24-capillary 3500xL Genetic Analyzers continue to set the standard in capillary electrophoresis.
The 3500xL Genetic Analyzer is a 24-capillary sequencing platform that can be used for a wide variety of applications, including de novo sequencing and resequencing (mutational profiling), as well as microsatellite analysis, MLPA™, LOH, MLST, and SNP validation or screening. The majority of applications can be run on a single polymer and capillary array.
Label or Dye 6-FAM, LIZ, NED, SID, TAZ, VIC Product Line GlobalFiler™ Product Type PCR Amplification Kit Shipping Condition Dry Ice Target Loci Amelogenin, CSF1PO, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D1S1656, D21S11, D22S1045, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, DYS391, FGA, IQC-Large, IQC-Small, SE33, TH01, TPOX, Y indel, vWA
The HID VeritiPro Thermal Cycler features a 96-well (0.2 mL) format with six independent temperature zones to provide precise temperature control and rapid temperature changes for conducting polymerase chain reactions (PCR). The HID VeritiPro Thermal Cycler features a powerful color touch screen and a USB port to simplify instrument setup and use.
The 24-capillary 3500xL Genetic Analyzer sets the standard in capillary electrophoresis. The 3500-series instruments are the first genetic analyzers designed with a specific feature set and workflow for the Human Identification application. The complete product combines the instrument with Applied Biosystems™ reagents, consumables and software, as well as industry leading support, to provide an integrated HID system solution that significantly improves ease-of-use and application efficiencies.
• Increased Consistency – Reduced signal variation with factory standardization and optional in-lane normalization
• Real-Time Data Quality Assessment – On instrument data evaluation features provide rapid identification and re-injection of samples requiring additional processing
• Improved Thermal Control – Smaller oven design and improved door seal for more consistent data migration
• HID Specific Workflow – Pre-configured for Applied Biosystems™ amplification kits to simplify run setup and software navigation
• Effortless Consumable Installation – Convenient one-time use consumable packaging
• Consumable Tracking with RFID Technology – On-instrument, real-time monitoring of consumable consumption and expiration
• Enhanced Throughput – Faster data generation with 8 or 24 capillary arrays and shorter run times
• Improved Polymer Pump Design – Reduced waste and fewer bubbles for better data quality
• Simplified Instrument Installation & Operation – New solid-state laser eliminates need for high voltage wiring and external venting
• Streamlined Instrument Maintenance – HID specific performance check and maintenance scheduling tools
• Data Chain of Custody – Controlled access and tracking through Security, Audit and eSig features
The 3500 analyzer is shipped with the following system components:
• Capillary Electrophoresis instrument.
• 3500 (8-capillary) array and POP™ polymer
• Human identification reagents and other consumables for system qualification.
• Dell™ computer workstation with flat-screen monitor.
• Integrated software for instrument control, data collection, quality control, and basecalling or sizing of samples.
NovaSeq X also significantly reduces waste and environmental impact, reflecting Illumina’s commitment to using its technology to support the health of people and the planet. NovaSeq X features a 90% reduction in packaging waste and weight and 50% reduction in plastic usage compared to NovaSeq 6000.
Model: Basic Yellow
Manufacturer: Lynn Peavey
Origin: United States