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Thermo Fisher Scientific
Instrument
Note: The Yfiler Plus kit is now approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.
The Yfiler Plus PCR Amplification Kit is designed for forensic laboratories that process highly challenging Y-STR casework and database samples. The Yfiler Plus kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA and offers laboratories both the power of discrimination and the performance to enable accurate results in less time from challenging samples.
The Yfiler Plus PCR Amplification Kit is a 6-dye multiplex assay for short tandem repeats (STRs) that allows amplification from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.
Key features of the Yfiler Plus PCR Amplification Kit:
• Improved discrimination capacity through new, highly discriminating markers
• Improved performance with degraded samples and PCR inhibitors
• Improved genotyping accuracy through expanded allelic ladder and virtual bins
• Concordance with existing data in reference haplotype databases
• Increased workflow efficiency enabling faster time-to-results than previous Y-STR kits
| Format | Kit |
| For Use With (Application) | Sequencing |
| Label or Dye | 6-FAM, LIZ, NED, SID, TAZ, VIC |
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No. of Reactions
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500 |
| Product Line | Yfiler™ |
| Product Type | Plus PCR Amplification Kit |
| Shipping Condition | Dry Ice |
| Target Loci | DYS438, DYS627, DYS458, DYS437, DYS391, DYS392, DYS635 (Y GATA C4), DYS19, DYS390, DYS439, DYS456, DYS393, DYS449, DYS387S1 a⁄b, DYS576, DYS460, DYS533, DYS389 I⁄II, DYS570, DYS385 a⁄b, DYS481, YGATA H4, DYS518, DYS448 |
Improved discrimination capacity
The Yfiler Plus PCR Amplification Kit enables multiplex amplification of the 17 Y-STR markers included in the AmpFLSTR Yfiler PCR Amplification Kits, as well as 10 new Y-STRs using 6-dye chemistry to offer the highest discriminatory capacity of any commercially available Y-STR kit. In particular, the inclusion of seven rapidly-mutating (RM) Y-STRs with mutation rates above 1 × 10(-2) helps improve resolution of paternal lineage differentiation as well as helps with discrimination of closely-related males.
More information from challenging casework samples
Y-STR analysis is useful for processing of complex casework samples with trace quantities of male DNA in presence of high level of female DNA. These samples may include rape kit samples, body fluid with few or no sperm present, and fingernail scrapings from female victims of violent assaults. These samples often show DNA degradation and contain inhibitors that can make the analysis difficult and lead to inconclusive results. The Yfiler Plus kit harnesses the latest developments in PCR amplification technology to help provide cleaner baseline and improved recovery of alleles with mixtures and highly inhibited samples.
Streamlined protocol and increased workflow efficiency
The Yfiler Plus kit provides faster thermal cycling conditions enabling shorter time to result. The kit is also designed to process single-source reference samples using direct PCR amplification from the following types of samples:
• Blood and buccal samples on treated paper substrates, without the need for sample purification
• Blood samples collected on untreated paper substrates and treated with Prep-n-Go Buffer
• Buccal samples collected on swab substrates and treated with Prep-n-Go Buffer
Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The Yfiler Plus kit is part of a comprehensively validated system for STR-based human identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.
The Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification with tower computer is the latest addition to our human identification (HID) real-time PCR family of products. This instrument is designed for both new and experienced users who need a simple, reliable, and affordable real-time PCR system that doesn’t require compromise on performance or quality. When used with our latest advances in quantification chemistry and HID Real-Time PCR Analysis Software, this system offers a sensitive, robust solution for forensic DNA quantification. It also helps prepare laboratories for future quantification technologies. With maximum dye versatility, this HID solution offers accurate, trusted results in a small benchtop footprint.
The Applied Biosystems SeqStudio Genetic Analyzer for HID is a low-throughput, easy-to-use, convenient benchtop system that delivers gold-standard fragment analysis or Sanger sequencing with just a simple click. It is validated across a broad range of HID forensic kits to deliver answers you can trust. The SeqStudio Genetic Analyzer couples the latest advancements in touchscreen usability with the same data quality, reliability, and support you have come to expect from Applied Biosystems instruments.
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A36187
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A36187
1 |
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A46208
Catalog Number:
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A46208
1 |
Amped Replay is an enhanced video player that allows investigators, frontline officers, first responders, security personnel and CCTV operators to conduct a quick first-level analysis of their videos, with easy conversion, quick corrections, redaction, and annotation functions, as well as a report, ideal for case building and disclosure. It includes Amped Software’s powerful DVR video conversion engine to view video without the need for other non-compatible, legacy, or proprietary player software. Its easy-to-use interface means no advanced skills are required to achieve professional results in the presentation of evidence, whether used as part of a case, forwarded to another unit or intelligence analysts, or sent to the media for identification. With Amped Replay, organizations are reassured that video is processed correctly at all levels, using validated forensic tools.
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Assisted Reproductive 
Prenatal screening 
New-born screening 
Screening 
Diagnosis 
Early detection 
Monitoring 
Illumina infectious diseases testing group 
GeneProof infectious diseases testing group 
Panagene infectious diseases testing group 
Digital forensics 
Inspection of Ballistics and Mechanical Traces 
Document examination 
Crime scene examination 
