Search
Search
Thermo Fisher Scientific
Instrument
Note: The Yfiler Plus kit is now approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.
The Yfiler Plus PCR Amplification Kit is designed for forensic laboratories that process highly challenging Y-STR casework and database samples. The Yfiler Plus kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA and offers laboratories both the power of discrimination and the performance to enable accurate results in less time from challenging samples.
The Yfiler Plus PCR Amplification Kit is a 6-dye multiplex assay for short tandem repeats (STRs) that allows amplification from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.
Key features of the Yfiler Plus PCR Amplification Kit:
• Improved discrimination capacity through new, highly discriminating markers
• Improved performance with degraded samples and PCR inhibitors
• Improved genotyping accuracy through expanded allelic ladder and virtual bins
• Concordance with existing data in reference haplotype databases
• Increased workflow efficiency enabling faster time-to-results than previous Y-STR kits
| Format | Kit |
| For Use With (Application) | Sequencing |
| Label or Dye | 6-FAM, LIZ, NED, SID, TAZ, VIC |
|
No. of Reactions
|
500 |
| Product Line | Yfiler™ |
| Product Type | Plus PCR Amplification Kit |
| Shipping Condition | Dry Ice |
| Target Loci | DYS438, DYS627, DYS458, DYS437, DYS391, DYS392, DYS635 (Y GATA C4), DYS19, DYS390, DYS439, DYS456, DYS393, DYS449, DYS387S1 a⁄b, DYS576, DYS460, DYS533, DYS389 I⁄II, DYS570, DYS385 a⁄b, DYS481, YGATA H4, DYS518, DYS448 |
Improved discrimination capacity
The Yfiler Plus PCR Amplification Kit enables multiplex amplification of the 17 Y-STR markers included in the AmpFLSTR Yfiler PCR Amplification Kits, as well as 10 new Y-STRs using 6-dye chemistry to offer the highest discriminatory capacity of any commercially available Y-STR kit. In particular, the inclusion of seven rapidly-mutating (RM) Y-STRs with mutation rates above 1 × 10(-2) helps improve resolution of paternal lineage differentiation as well as helps with discrimination of closely-related males.
More information from challenging casework samples
Y-STR analysis is useful for processing of complex casework samples with trace quantities of male DNA in presence of high level of female DNA. These samples may include rape kit samples, body fluid with few or no sperm present, and fingernail scrapings from female victims of violent assaults. These samples often show DNA degradation and contain inhibitors that can make the analysis difficult and lead to inconclusive results. The Yfiler Plus kit harnesses the latest developments in PCR amplification technology to help provide cleaner baseline and improved recovery of alleles with mixtures and highly inhibited samples.
Streamlined protocol and increased workflow efficiency
The Yfiler Plus kit provides faster thermal cycling conditions enabling shorter time to result. The kit is also designed to process single-source reference samples using direct PCR amplification from the following types of samples:
• Blood and buccal samples on treated paper substrates, without the need for sample purification
• Blood samples collected on untreated paper substrates and treated with Prep-n-Go Buffer
• Buccal samples collected on swab substrates and treated with Prep-n-Go Buffer
Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The Yfiler Plus kit is part of a comprehensively validated system for STR-based human identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
The ProFlex 96-well PCR system is the latest in our line of Applied Biosystems thermal cyclers. The ProFlex system combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.
The ProFlex 96-well PCR system allows you to:
• Interchange the 96-well 0.2-mL block with another block type if throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate an old instrument with Thermal Simulation modes
• Optimize PCR quickly and efficiently with better-than-gradient VeriFlex blocks
The Applied Biosystems VeritiPro Thermal Cycler delivers proven reliability with advanced temperature control technology and connectivity. Take advantage of next-level PCR technology featuring faster ramping speeds and lower noise output. Connect to the cloud-enabled VeritiPro Thermal Cycler remotely. Conveniently design and securely upload your methods, monitor runs, and check instrument availability from any mobile device or desktop computer using Connect, our cloud-based platform.
The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.
This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.
The Applied Biosystems SeqStudio Genetic Analyzer for HID is a low-throughput, easy-to-use, convenient benchtop system that delivers gold-standard fragment analysis or Sanger sequencing with just a simple click. It is validated across a broad range of HID forensic kits to deliver answers you can trust. The SeqStudio Genetic Analyzer couples the latest advancements in touchscreen usability with the same data quality, reliability, and support you have come to expect from Applied Biosystems instruments.
|
A36187
Catalog Number:
Unit Size:
Qty:
|
A36187
1 |
|
A46208
Catalog Number:
Unit Size:
Qty:
|
A46208
1 |
The 24-capillary 3500xL Genetic Analyzer sets the standard in capillary electrophoresis. The 3500-series instruments are the first genetic analyzers designed with a specific feature set and workflow for the Human Identification application. The complete product combines the instrument with Applied Biosystems™ reagents, consumables and software, as well as industry leading support, to provide an integrated HID system solution that significantly improves ease-of-use and application efficiencies.
• Increased Consistency – Reduced signal variation with factory standardization and optional in-lane normalization
• Real-Time Data Quality Assessment – On instrument data evaluation features provide rapid identification and re-injection of samples requiring additional processing
• Improved Thermal Control – Smaller oven design and improved door seal for more consistent data migration
• HID Specific Workflow – Pre-configured for Applied Biosystems™ amplification kits to simplify run setup and software navigation
• Effortless Consumable Installation – Convenient one-time use consumable packaging
• Consumable Tracking with RFID Technology – On-instrument, real-time monitoring of consumable consumption and expiration
• Enhanced Throughput – Faster data generation with 8 or 24 capillary arrays and shorter run times
• Improved Polymer Pump Design – Reduced waste and fewer bubbles for better data quality
• Simplified Instrument Installation & Operation – New solid-state laser eliminates need for high voltage wiring and external venting
• Streamlined Instrument Maintenance – HID specific performance check and maintenance scheduling tools
• Data Chain of Custody – Controlled access and tracking through Security, Audit and eSig features
Amped Replay is an enhanced video player that allows investigators, frontline officers, first responders, security personnel and CCTV operators to conduct a quick first-level analysis of their videos, with easy conversion, quick corrections, redaction, and annotation functions, as well as a report, ideal for case building and disclosure. It includes Amped Software’s powerful DVR video conversion engine to view video without the need for other non-compatible, legacy, or proprietary player software. Its easy-to-use interface means no advanced skills are required to achieve professional results in the presentation of evidence, whether used as part of a case, forwarded to another unit or intelligence analysts, or sent to the media for identification. With Amped Replay, organizations are reassured that video is processed correctly at all levels, using validated forensic tools.
Model: Amido Black
Manufacturer: Lynn Peavey
Origin: United States
Model: Basic Yellow
Manufacturer: Lynn Peavey
Origin: United States
Model: Crystal Violet Powder
Manufacturer: Lynn Peavey
Origin: United States
Assisted Reproductive 
Prenatal screening 
New-born screening 
Screening 
Diagnosis 
Early detection 
Monitoring 
Illumina infectious diseases testing group 
GeneProof infectious diseases testing group 
Panagene infectious diseases testing group 
Digital forensics 
Inspection of Ballistics and Mechanical Traces 
Document examination 
Crime scene examination 
