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Thermo Fisher Scientific
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Note: The Yfiler Plus kit is now approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.
The Yfiler Plus PCR Amplification Kit is designed for forensic laboratories that process highly challenging Y-STR casework and database samples. The Yfiler Plus kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA and offers laboratories both the power of discrimination and the performance to enable accurate results in less time from challenging samples.
The Yfiler Plus PCR Amplification Kit is a 6-dye multiplex assay for short tandem repeats (STRs) that allows amplification from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.
Key features of the Yfiler Plus PCR Amplification Kit:
• Improved discrimination capacity through new, highly discriminating markers
• Improved performance with degraded samples and PCR inhibitors
• Improved genotyping accuracy through expanded allelic ladder and virtual bins
• Concordance with existing data in reference haplotype databases
• Increased workflow efficiency enabling faster time-to-results than previous Y-STR kits
| Format | Kit |
| For Use With (Application) | Sequencing |
| Label or Dye | 6-FAM, LIZ, NED, SID, TAZ, VIC |
|
No. of Reactions
|
500 |
| Product Line | Yfiler™ |
| Product Type | Plus PCR Amplification Kit |
| Shipping Condition | Dry Ice |
| Target Loci | DYS438, DYS627, DYS458, DYS437, DYS391, DYS392, DYS635 (Y GATA C4), DYS19, DYS390, DYS439, DYS456, DYS393, DYS449, DYS387S1 a⁄b, DYS576, DYS460, DYS533, DYS389 I⁄II, DYS570, DYS385 a⁄b, DYS481, YGATA H4, DYS518, DYS448 |
Improved discrimination capacity
The Yfiler Plus PCR Amplification Kit enables multiplex amplification of the 17 Y-STR markers included in the AmpFLSTR Yfiler PCR Amplification Kits, as well as 10 new Y-STRs using 6-dye chemistry to offer the highest discriminatory capacity of any commercially available Y-STR kit. In particular, the inclusion of seven rapidly-mutating (RM) Y-STRs with mutation rates above 1 × 10(-2) helps improve resolution of paternal lineage differentiation as well as helps with discrimination of closely-related males.
More information from challenging casework samples
Y-STR analysis is useful for processing of complex casework samples with trace quantities of male DNA in presence of high level of female DNA. These samples may include rape kit samples, body fluid with few or no sperm present, and fingernail scrapings from female victims of violent assaults. These samples often show DNA degradation and contain inhibitors that can make the analysis difficult and lead to inconclusive results. The Yfiler Plus kit harnesses the latest developments in PCR amplification technology to help provide cleaner baseline and improved recovery of alleles with mixtures and highly inhibited samples.
Streamlined protocol and increased workflow efficiency
The Yfiler Plus kit provides faster thermal cycling conditions enabling shorter time to result. The kit is also designed to process single-source reference samples using direct PCR amplification from the following types of samples:
• Blood and buccal samples on treated paper substrates, without the need for sample purification
• Blood samples collected on untreated paper substrates and treated with Prep-n-Go Buffer
• Buccal samples collected on swab substrates and treated with Prep-n-Go Buffer
Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The Yfiler Plus kit is part of a comprehensively validated system for STR-based human identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.
The Applied Biosystems HID NIMBUS Presto System is an easy-to-use, compact instrument that combines the KingFisher Presto Purification System with an industry leading automated liquid handling workstation to precisely purify nucleic acids from lysed samples for use in sensitive downstream analyses such as DNA quantification and STR amplification.
The Applied Biosystems VeritiPro Thermal Cycler delivers proven reliability with advanced temperature control technology and connectivity. Take advantage of next-level PCR technology featuring faster ramping speeds and lower noise output. Connect to the cloud-enabled VeritiPro Thermal Cycler remotely. Conveniently design and securely upload your methods, monitor runs, and check instrument availability from any mobile device or desktop computer using Connect, our cloud-based platform.
The 24-capillary 3500xL Genetic Analyzer sets the standard in capillary electrophoresis. The 3500-series instruments are the first genetic analyzers designed with a specific feature set and workflow for the Human Identification application. The complete product combines the instrument with Applied Biosystems™ reagents, consumables and software, as well as industry leading support, to provide an integrated HID system solution that significantly improves ease-of-use and application efficiencies.
• Increased Consistency – Reduced signal variation with factory standardization and optional in-lane normalization
• Real-Time Data Quality Assessment – On instrument data evaluation features provide rapid identification and re-injection of samples requiring additional processing
• Improved Thermal Control – Smaller oven design and improved door seal for more consistent data migration
• HID Specific Workflow – Pre-configured for Applied Biosystems™ amplification kits to simplify run setup and software navigation
• Effortless Consumable Installation – Convenient one-time use consumable packaging
• Consumable Tracking with RFID Technology – On-instrument, real-time monitoring of consumable consumption and expiration
• Enhanced Throughput – Faster data generation with 8 or 24 capillary arrays and shorter run times
• Improved Polymer Pump Design – Reduced waste and fewer bubbles for better data quality
• Simplified Instrument Installation & Operation – New solid-state laser eliminates need for high voltage wiring and external venting
• Streamlined Instrument Maintenance – HID specific performance check and maintenance scheduling tools
• Data Chain of Custody – Controlled access and tracking through Security, Audit and eSig features
Designed using capillary electrophoresis technology trusted by HID labs for over 25 years, the Applied Biosystems SeqStudio 24 Flex Genetic Analyzer for Human Identification with 24-capillary array delivers the flexibility and accuracy your work demands. Innovative capabilities, including a continuously accessible four-plate deck, can help you achieve reliable STR results efficiently. Features such as lab-friendly connectivity and easy, on-instrument training videos can help get you up and running quickly and minimize downtime.
Increased lab productivity
The SeqStudio 24 Flex Genetic Analyzer for HID is the genetic analyzer for HID fragment analysis and Sanger sequencing that delivers the high-quality data and reliable performance that forensic scientists expect, with design enhancements for improved flexibility, usability, connectivity, and serviceability.
Flexibility
• Accessibility—continuously load plates and reprioritize urgent samples without having to terminate the current run
• Increased walk-away capacity—four plate positions accommodating 8-tube strips and 96-well sample plates
• Work when and where you want—set up your plate and monitor run progress from anywhere using the Thermo Fisher Connect Platform.
Usability
• Designed for all levels of experience—simple one-button startup, autocalibration, and onscreen step by-step routine maintenance instructions and onboard self-help training videos
• Simplified capillary array installation—redesigned, more robust arrays with “click-and-slide” retractor tab and enhanced detection cell housing provide improved capillary protection, ease of handling, insertion, and positioning • Compatibility with Applied Biosystems 3500 Series genetic analyzer buffers and polymers
• Easy inventory management with radio-frequency identification (RFID)–enabled tracking of consumables usage
• No desktop computer required—integrated touchscreen computer, intuitive software, walk-through wizards, and Plate Manager 2.1 Software for simplified plate setup and instrument operation
Connectivity
Integrate the communication technologies and security options of today to propel laboratory efficiencies of tomorrow.
• Connect your way—integrate your instrument via local area network (LAN), Wi-Fi, USB ports, and LIMS compatibility
• Confidently walk away—monitor your run and data via the Connect Platform from your mobile phone, tablet, laptop, or remote desktop
• Data integrity and compliance—optional security, audit, and electronic signature (SAE) software for an electronic chain of custody
Serviceability
Applied Biosystems instrument innovation continues with additionally included digital service ecosystem for remote troubleshooting.*
• Reduced service visits—70% of issues remotely resolved
• Reduced instrument downtime—remotely resolve issues in minutes, not days, using onboard Smart Help and Remote Support features
• Real-time audio/video collaboration—the Remote Support feature brings our technicians into your laboratory using cutting-edge, security-minded digital tools
• Secure transfer of instrument performance data—send log and run files directly from the instrument with the push of a button using Smart Help
*Connectivity required for Smart Help and Remote Monitoring
Amped Authenticate is a software application for forensic image authentication, tampering detection, and camera ballistics on digital images. This product is the only image authentication software on the market that offers a complete suite of powerful tools to exploit the data behind digital images, allowing for the analysis of image integrity, authenticity, metadata, source and history, and detection of tampering prior to its use as intelligence and evidence. With a single product, several tests can be performed to determine if an image has been manipulated as well as verify if a photo was taken from a specific device.
The SL-450 is ruggedly portable and cable-free while allowing users to move unhindered around any crime scene location. Its powerful 35W Xenon lamp is powered by a lithium-ion battery that provides 90 minutes of continuous power.
SUITCASE SCENE EXAMINATION VERSATILE
Model: 56031
Manufacturer: Lynn Peavey
Origin: United States
Model: Army Compact Kit
Manufacturer: Lynn Peavey
Origin: United States
Assisted Reproductive 
Prenatal screening 
New-born screening 
Screening 
Diagnosis 
Early detection 
Monitoring 
Illumina infectious diseases testing group 
GeneProof infectious diseases testing group 
Panagene infectious diseases testing group 
Digital forensics 
Inspection of Ballistics and Mechanical Traces 
Document examination 
Crime scene examination 
