Certificate

Brand

Thermo Fisher Scientific

Product Type

Instrument

Highlights

The Applied Biosystems VeriFiler Plus PCR Amplification Kit is a highly discriminating, highly sensitive, robust kit optimized for forensic casework. Utilizing our 6-dye chemistry, the VeriFiler Plus kit targets 25 markers: 23 autosomal STRs, including Penta D, Penta E, and two gender discrimination markers. These markers meet all of the requirements CODIS markers  as well as the Chinese National autosomal DNA database. The VeriFiler Plus kit was specifically developed to maximize sensitivity to enable maximum information recovery from challenging casework samples including touch, degraded, and inhibited samples, yet can also be run in direct mode for single source reference samples in database workflows. The VeriFiler Plus kit includes all of the same markers as the VeriFiler Express and Huaxia Platinum kits, but in a complementary layout to maximize discrimination for degraded samples. The VeriFiler Plus kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. As with all Applied Biosystems STR kits, the VeriFiler Plus kit is ISO18385 Forensic DNA Grade, giving you piece of mind when using this kit for your casework samples.

Application

Key features of the VeriFiler Plus PCR Amplification Kit include:
• Very high discrimination powers across global populations
• Inclusion of all CODIS- and Chinese National database loci-required markers
• One kit for both casework and database applications
• Concordance with Huaxia Platinum and VeriFiler Express kits; the VeriFiler Plus kit contains the same loci as these databasing kits
• High sensitivity enabled by a high-volume sample load (up to 17.5 µL)
• 11 mini-STRs maximize information recovery from degraded DNA samples
• Internal Quality Control (IQC) system enables detection of and discrimination between amplification inhibition and DNA sample degradation, providing actionable information regarding further sample processing or testing
• Gender confirmation through two Y-chromosome markers
• Efficient workflow with 76-minute PCR cycle time

Specifications

Format Tube
Label or Dye VIC
Product Line VeriFiler
Product Type PCR Amplification Kit
Shipping Condition Dry Ice
Target Loci AMEL, IQCL, IQCS, Penta D, Penta E, Yindel, D18S51, D5S818, D16S539, D3S1358, D2S1338, TPOX, FGA, D1S1656, D10S1248, D7S820, D13S317, CSF1PO, D22S1045, D6S1043, TH01, D2S441, D8S1179, vWA, D19S433, D12S391, D21S11

Other information

Casework kit with loci matching DNA profiles in databases
The VeriFiler Plus PCR Amplification Kit was developed to offer the highest level of compatibility between DNA databases and the casework samples genotyped with this kit. Specifically, the VeriFiler Plus kit amplifies all of the loci that are included in the DNA profiles used to build important national and local databases, including the 20 core loci proposed by the Chinese Ministry of Public Security. All required CODIS markers and the highly discriminatory Penta D and Penta E markers are included as well. With 25 markers, the VeriFiler Plus kit offers extremely high discrimination power, enabling matching of casework samples with database profiles with the highest level of confidence.

Exceptional sensitivity enables recovery of more alleles
Developed specifically for casework applications, the VeriFiler Plus PCR Amplification Kit provides DNA analysts with the capability to recover more information from casework samples. The higher sample load volume (up to 17.5 µL) and the inclusion of 11 mini-STRs, as defined by amplicons less than 250 base pairs, provide the highest chance of allele detection. As crime laboratories move toward using DNA forensics in volume crimes, detection of profiles or even partial profiles from samples with a low amount of DNA becomes increasingly important. With an optimized buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples, the VeriFiler Plus kit delivers the capability to provide maximum possible information even from the most challenging samples.





Efficiency for casework or databasing applications
In addition to high performance casework, the VeriFiler Plus kit has also been validated with multiple sample collection devices such as treated paper, untreated paper, and swabs for direct amplification of single source samples. This dual-use kit enables labs to more efficiently manage their inventory and analysis workflows.


IQC system for more informative results and better decision guidance
The VeriFiler Plus PCR Amplification Kit includes an IQC system that consists of two exogenous, synthetic DNA sequences with primers specific for each IQC target that help distinguish DNA sample degradation from amplification reaction inhibition. In addition, the IQC system serves as a positive control for PCR amplification. The IQC system results, together with an assessment of a DNA profile’s electropherogram, provide DNA analysts with confirmation of inhibition or inferred degradation by virtue of a lack of inhibition, providing data needed to make decisions on additional steps that can be taken to extract more information from a sample.

Experience matters
Thermo Fisher Scientific is the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

We also offer a range of Human Identification Professional Services (HPS) to assist with bringing new kits and workflows online. Since 2007, the HPS team has completed over 400 successful validation projects worldwide with a staff of more than 20 technical support specialists, each averaging eight years of real-world forensic experience, providing customers with in-depth training and support on our instruments, chemistries, and software.

Related Products

GlobalFiler™ PCR Amplification Kit

The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.

ProFlex™ 96-well PCR System

The ProFlex 96-well PCR system is the latest in our line of Applied Biosystems thermal cyclers. The ProFlex system combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.

The ProFlex 96-well PCR system allows you to:
• Interchange the 96-well 0.2-mL block with another block type if throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate an old instrument with Thermal Simulation modes
• Optimize PCR quickly and efficiently with better-than-gradient VeriFlex blocks

QuantStudio™ 5 Real-Time PCR System for Human Identification, 96-well, 0.2 mL, desktop

The Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification with tower computer is the latest addition to our human identification (HID) real-time PCR family of products. This instrument is designed for both new and experienced users who need a simple, reliable, and affordable real-time PCR system that doesn’t require compromise on performance or quality. When used with our latest advances in quantification chemistry and HID Real-Time PCR Analysis Software, this system offers a sensitive, robust solution for forensic DNA quantification. It also helps prepare laboratories for future quantification technologies. With maximum dye versatility, this HID solution offers accurate, trusted results in a small benchtop footprint.

Contents & Storage

Desktop Computer

7500 Real-Time PCR System for Human Identification with Dell Notebook D520

The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.

This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.

SeqStudio™ Genetic Analyzer for HID, with training

The Applied Biosystems SeqStudio Genetic Analyzer for HID is a low-throughput, easy-to-use, convenient benchtop system that delivers gold-standard fragment analysis or Sanger sequencing with just a simple click. It is validated across a broad range of HID forensic kits to deliver answers you can trust. The SeqStudio Genetic Analyzer couples the latest advancements in touchscreen usability with the same data quality, reliability, and support you have come to expect from Applied Biosystems instruments.

Package Components

A36187
Catalog Number:

Unit Size:
Qty:
A36187

1

A46208
Catalog Number:

Unit Size:
Qty:
A46208

1

Batch Convert Unplayable Videos

Overview

Amped DVRConv is the easiest way to automatically batch convert unplayable video files. Videos from proprietary CCTV/DVR systems, dashcams, body worn cameras, and any other video source can now be converted without effort. With Amped DVRConv, you just drag, drop, and you’re done. This is an ideal tool for technicians tasked with converting a great number of surveillance videos in various proprietary formats, speeding up the triage in cases such as major investigations.

Army Compact Kit-equipment for field operations to wear feet

Product Information

Model: Army Compact Kit

Manufacturer: Lynn Peavey

Origin: United States

Peavey-Print: Super Glue™

Product Information

Model: Super Glue

Manufacturer: Lynn Peavey

Origin: United States

Fold-A-Lab kit-portable sauna cabinet

Product Information

Model: Original Fold-A-Lab Kit

Manufacturer: Lynn Peavey

Origin: United States

Fuming Hot Box

Product Information

Model: Fuming Hot Box

Manufacturer: Lynn Peavey

Origin: United States