Certificate

Brand

Thermo Fisher Scientific

Product Type

Instrument

Highlights

The VeriFiler Express PCR Amplification Kit is a direct-amplification STR kit that combines a high power of discrimination with superior performance and fast time to results. The VeriFiler Express kit enables multiplex amplification of the 22 STR loci included in the GlobalFiler Express kit, as well the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.

Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.

Application

Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
    –Buccal samples on treated and untreated paper
    –Buccal samples on cotton swab
    –Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins

Specifications

Product Line VeriFiler
Label or Dye VIC
Product Type VeriFiler Express PCR Amplification Kit

 

Contents & Storage

• 2 x 1 mL VeriFiler Express Primer Set
• 2 x 1 mL VeriFiler Express Master Mix
• 1 x 50 µL of 2 ng/µL VeriFiler Express DNA Control 007
• 1 x 50 µL VeriFiler Express Allelic Ladder

Store at –15 to –25°C on receipt, 2 to 8°C after initial use.

Other information

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).

Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.

Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.

Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.

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• Secure transfer of instrument performance data—send log and run files directly from the instrument with the push of a button using Smart Help

*Connectivity required for Smart Help and Remote Monitoring

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Designed using capillary electrophoresis technology trusted by HID labs for over 25 years, the Applied Biosystems SeqStudio 24 Flex Genetic Analyzer for Human Identification with 24-capillary array delivers the flexibility and accuracy your work demands. Innovative capabilities, including a continuously accessible four-plate deck, can help you achieve reliable STR results efficiently. Features such as lab-friendly connectivity and easy, on-instrument training videos can help get you up and running quickly and minimize downtime.

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The SeqStudio 24 Flex Genetic Analyzer for HID is the genetic analyzer for HID fragment analysis and Sanger sequencing that delivers the high-quality data and reliable performance that forensic scientists expect, with design enhancements for improved flexibility, usability, connectivity, and serviceability.

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• Accessibility—continuously load plates and reprioritize urgent samples without having to terminate the current run
• Increased walk-away capacity—four plate positions accommodating 8-tube strips and 96-well sample plates
• Work when and where you want—set up your plate and monitor run progress from anywhere using the Thermo Fisher Connect Platform.

Usability
• Designed for all levels of experience—simple one-button startup, autocalibration, and onscreen step by-step routine maintenance instructions and onboard self-help training videos
• Simplified capillary array installation—redesigned, more robust arrays with “click-and-slide” retractor tab and enhanced detection cell housing provide improved capillary protection, ease of handling, insertion, and positioning • Compatibility with Applied Biosystems 3500 Series genetic analyzer buffers and polymers
• Easy inventory management with radio-frequency identification (RFID)–enabled tracking of consumables usage
• No desktop computer required—integrated touchscreen computer, intuitive software, walk-through wizards, and Plate Manager 2.1 Software for simplified plate setup and instrument operation

Connectivity
Integrate the communication technologies and security options of today to propel laboratory efficiencies of tomorrow.
• Connect your way—integrate your instrument via local area network (LAN), Wi-Fi, USB ports, and LIMS compatibility
• Confidently walk away—monitor your run and data via the Connect Platform from your mobile phone, tablet, laptop, or remote desktop
• Data integrity and compliance—optional security, audit, and electronic signature (SAE) software for an electronic chain of custody

Serviceability
Applied Biosystems instrument innovation continues with additionally included digital service ecosystem for remote troubleshooting.*
• Reduced service visits—70% of issues remotely resolved
• Reduced instrument downtime—remotely resolve issues in minutes, not days, using onboard Smart Help and Remote Support features
• Real-time audio/video collaboration—the Remote Support feature brings our technicians into your laboratory using cutting-edge, security-minded digital tools
• Secure transfer of instrument performance data—send log and run files directly from the instrument with the push of a button using Smart Help

*Connectivity required for Smart Help and Remote Monitoring

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