VeriFiler™ Express PCR Amplification Kit - BIOMEDIC JSC

Home

Products & Applications

Forensics

VeriFiler™ Express PCR Amplification Kit

Certificate

Brand

Thermo Fisher Scientific

Product Type

Instrument

Highlights

The VeriFiler Express PCR Amplification Kit is a direct-amplification STR kit that combines a high power of discrimination with superior performance and fast time to results. The VeriFiler Express kit enables multiplex amplification of the 22 STR loci included in the GlobalFiler Express kit, as well the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.

Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.

Application

Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
    –Buccal samples on treated and untreated paper
    –Buccal samples on cotton swab
    –Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins

Specifications

Product Line	VeriFiler
Label or Dye	VIC
Product Type	VeriFiler Express PCR Amplification Kit

Contents & Storage

• 2 x 1 mL VeriFiler Express Primer Set
• 2 x 1 mL VeriFiler Express Master Mix
• 1 x 50 µL of 2 ng/µL VeriFiler Express DNA Control 007
• 1 x 50 µL VeriFiler Express Allelic Ladder

Store at –15 to –25°C on receipt, 2 to 8°C after initial use.

Other information

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).

Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.

Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.

Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.

Related Products

GlobalFiler™ PCR Amplification Kit

The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.

HID Nimbus™ Presto System

The Applied Biosystems HID NIMBUS Presto System is an easy-to-use, compact instrument that combines the KingFisher Presto Purification System with an industry leading automated liquid handling workstation to precisely purify nucleic acids from lysed samples for use in sensitive downstream analyses such as DNA quantification and STR amplification.

ProFlex™ 96-well PCR System

The ProFlex 96-well PCR system is the latest in our line of Applied Biosystems thermal cyclers. The ProFlex system combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.

The ProFlex 96-well PCR system allows you to:
• Interchange the 96-well 0.2-mL block with another block type if throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate an old instrument with Thermal Simulation modes
• Optimize PCR quickly and efficiently with better-than-gradient VeriFlex blocks

VeritiPro™ Thermal Cycler, 384-well

The Applied Biosystems VeritiPro Thermal Cycler delivers proven reliability with advanced temperature control technology and connectivity. Take advantage of next-level PCR technology featuring faster ramping speeds and lower noise output. Connect to the cloud-enabled VeritiPro Thermal Cycler remotely. Conveniently design and securely upload your methods, monitor runs, and check instrument availability from any mobile device or desktop computer using Connect, our cloud-based platform.

QuantStudio™ 5 Real-Time PCR System for Human Identification, 96-well, 0.2 mL, desktop

The Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification with tower computer is the latest addition to our human identification (HID) real-time PCR family of products. This instrument is designed for both new and experienced users who need a simple, reliable, and affordable real-time PCR system that doesn’t require compromise on performance or quality. When used with our latest advances in quantification chemistry and HID Real-Time PCR Analysis Software, this system offers a sensitive, robust solution for forensic DNA quantification. It also helps prepare laboratories for future quantification technologies. With maximum dye versatility, this HID solution offers accurate, trusted results in a small benchtop footprint.

Contents & Storage

Desktop Computer

7500 Real-Time PCR System for Human Identification with Dell Notebook D520

The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.

This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.

SL-450 Hand-held crime scene light source

The SL-450 is ruggedly portable and cable-free while allowing users to move unhindered around any crime scene location. Its powerful 35W Xenon lamp is powered by a lithium-ion battery that provides 90 minutes of continuous power.

Hot Shot™ Cyanoacrylate Fuming

Product Information

Model: HotShot Fingerprint Developer

Manufacturer: Lynn Peavey

Origin: United States

Peavey-Print: Super Glue™

Product Information

Model: Super Glue

Manufacturer: Lynn Peavey

Origin: United States

Fuming Hot Box

Product Information

Model: Fuming Hot Box

Manufacturer: Lynn Peavey

Origin: United States

Obstetrics & Gynecology

Oncology

Infectious diseases

Forensics

Obstetrics & Gynecology

Oncology

Infectious diseases

Forensics