TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of soma in NGS – Next Generation Sequencing of Illumina


Illumina
Reagents
26 Variants detection from Somatic cells
TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of soma in NGS – Next Generation Sequencing of Illumina
TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of soma in NGS – Next Generation Sequencing of Illumina
| List | Parameter |
| Time preparation | Total <7h (including, 2.5h to manual) |
| Sequencing time | 22h in MiSeq system, upto 33h in HiSeq 2500 system |
| Technology | Next Generation Sequencing |
| Input sample | Based on QC, typically appx. 30-300 ng DNA |
| Sequencing | 2 × 121bp |
| 15 gene được giải trình tự | AKT1, ALK, APC, NRAS, BRAF, CDH1, CTNNB1, EGFR,
ERBB2, FBXW7, FGFR2, FOXL2, GNAQ, GNAS, KIT, KRAS, MAP2K1, MET, MSH6, NRAS, PDGFRA, PIK3CA, PTEN, SMAD4, SRC, STK11, TP53 |
| Amplicon | Include 174 amplicon with average size 165-195 bp |
| Sample | FFPE |
| Compatible NGS system | MiniSeq, MiSeq, MiSeqDx ở chế độ Reseach Mode, NextSeq 550 |
| Sample running capacity | 4 sample/time on MiSeq48 sample running on HiSeq 2500 (rapid-run mode) |
| Limit detection | 3% |
| Frequency | ≤5% |
| Coverage | Min 1000×, avg.7000× |
| Variants detection | Indels, changing of genetics in Somatic cells |
Supplier
Illumina


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TruSight Tumor 26 Kit includes library preparation reagent set and uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 26 genes that are commonly related to mutations in solid tumors and somatic variants