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Next-Generation Sequencing System – NextSeq 2000

Certificate

Brand

Illumina

Product Type

Reagents

Highlights

26 Variants detection from Somatic cells

TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of  soma in NGS – Next Generation Sequencing of Illumina

Application

TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of  soma in NGS – Next Generation Sequencing of Illumina

Specifications

List Parameter
Time preparation Total <7h (including, 2.5h to manual)
Sequencing time 22h in MiSeq system, upto 33h in HiSeq 2500 system
Technology  Next Generation Sequencing
Input sample Based on QC, typically appx. 30-300 ng DNA
Sequencing 2 × 121bp
15 gene được giải trình tự AKT1, ALK, APC, NRAS, BRAF, CDH1, CTNNB1, EGFR, 

ERBB2, FBXW7, FGFR2, FOXL2, GNAQ, GNAS, KIT, KRAS, MAP2K1, MET, MSH6, NRAS, PDGFRA, PIK3CA, PTEN, SMAD4, SRC, STK11, TP53
Amplicon Include 174 amplicon with average size 165-195 bp
Sample  FFPE
Compatible NGS system MiniSeq, MiSeq, MiSeqDx ở chế độ Reseach Mode, NextSeq 550
Sample running capacity 4 sample/time on MiSeq48 sample running on HiSeq 2500 (rapid-run mode)
Limit detection 3%
Frequency ≤5%
Coverage Min 1000×, avg.7000×
Variants detection Indels, changing of genetics in Somatic cells

Other information

Supplier

Illumina

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Zymot ICSI

Next-Generation Sequencing System – NextSeq 2000