The ZymoBIOMICS Fecal Reference with TruMatrix™ Technology is a microbial reference material composed of stool from healthy donors for quality control, process validation, assay development, and proficiency testing. The fecal reference was homogenized in one large batch to produce consistency in every vial, eliminating typical lot-to-lot variability. With over 2 million preps available, this reference is intended to be used as a control for workflow performance, as well as comparing workflow consistency across replicates, studies, and laboratories. To facilitate methods comparison, a platform is in development to enable users to compare characterization data against applied methodology.

16S rRNA sequencing is a routine technique for microbiome composition profiling. Compared to shotgun metagenomics sequencing, 16S rRNA sequencing is more cost-effective and more robust; it generally requires less input DNA and is less impacted by the presence of host DNA. However, 16S rRNA sequencing has its own challenges. One major challenge is the formation of PCR chimeric sequences, which are artificial sequences resulting from the recombination of two or more PCR templates. Additionally, with common 16S primers, it is difficult to achieve both species-level resolution and broad phylogenetic coverage. Moreover, common 16S library preparation protocols used in the field have not been optimized to be cost-effective for large-scale applications. The Quick-16S NGS Library Prep Kit aims to standardize the library preparation process for 16S rRNA sequencing. Distinguishing features of the kit are described below. Fastest 16S rRNA Library Prep. The Quick-16S NGS Library Prep Kit utilizes real-time (quantitative) PCR (qPCR) rather than endpoint PCR for 16S rRNA amplification, enabling direct quantification of PCR products and eliminating the need for additional library quantification analysis such as TapeStation analysis or gel electrophoresis. An enzymatic clean-up is introduced between the two PCR steps, saving time and reducing costs as compared to lengthy AMPure bead-based clean-ups. With these features, the kit dramatically reduces the hands-on time of 16S library preparation. Simple. The Quick-16S NGS Library Prep Kit includes all the reagents needed to convert 96 DNA samples to a 16S library. The resulting library is directly compatible with the Illumina MiSeq without needing additional custom sequencing primers. Accurate. The utilization of real-time PCR also enables users to control PCR cycles. This limits chimera formation and PCR bias while obtaining enough products for subsequent sequencing. In most cases, the abundance of PCR chimeric sequences is maintained below 2%. Increased Coverage. Due to the rapid expansion of 16S rRNA databases, the insufficient microbial coverage of common 16S primer sets has become evident. Zymo Research has re-designed two common primer sets targeting the 16S V1-V2 and 16S V3-V4 regions based on the most updated 16S reference database and significantly improved their coverage.

The Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure^® bead-based clean-ups. Additional library quantification analysis such as TapeStation^® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

The EntroGen NGS Targeted Hotspot Panel is a comprehensive assay that detects clinically relevant mutations in solid tumors using next-generation sequencing, compatible with fresh frozen and FFPE samples. It offers high sensitivity, low DNA input, and the ability to batch up to 12 samples in a single run, making it ideal for labs with limited sample volumes.

MORE INFORMATION FROM ONE REACTION

• PCR kit detects and distinguishes Herpes Simplex Virus 1 and Herpes Simplex Virus 2 in a single reaction tube

EXCELLENT QUALITY

• The highest possible diagnostic sensitivity (100.00%) and diagnostic specificity (97.62%)

SIMPLE LABORATORY WORKFLOW

• Easily combinable with other GeneProof PCR kits in one workflow

DETECTION OF ALL HUMAN ADENOVIRUS SPECIES

The kit enables detection of Adenovirus respiratory species A–D, G and gastrointestinal species E-F

WIDE RANGE OF VALIDATED CLINICAL MATERIALS

• Validated specimen: aspirate, plasma, stool, swab, urine, whole blood
• Enables detection of both respiratory and gastrointestinal samples

Certificate: ROU 

Supplier: Illumina 

For Mid and Low-throughput laboratories performing COVID-19 surveillance and identification of new variants

BRCAaccuTest™ PLUS with CE-IVD certificate for HBOC Breast/Ovarian Cancer Test

The PANA RealTyper™ STD Kit is an in vitro diagnostic reagent for genotyping of 13 pathogens involved in sexually transmitted infections (STI) from vaginal swab and urine.

The PANA RealTyper™ STD Kit is a CE marked diagnostic device in accordance with the European Union in vitro Diagnostic Medical Device Directive 98/79/EC.

TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of  soma in NGS – Next Generation Sequencing of Illumina