Thermo Fisher Scientific
Instrument
For Research Use Only
The 3500 analyzer is shipped with the following system components:
• Capillary Electrophoresis instrument.
• 3500 (8-capillary) array and POP™ polymer
• Human identification reagents and other consumables for system qualification.
• Dell™ computer workstation with flat-screen monitor.
• Integrated software for instrument control, data collection, quality control, and basecalling or sizing of samples.
Type | Genetic Analyzer |
For Use With (Equipment) | 3500 Genetic Analyzer |
Analysis Time | 30 to 140 min. (varies depending on run module) |
Throughput | Range: 80 to 368 sequencing samples per day (varies depending on run module), ≥312 samples per day (HID36 POP4 Module), ≥ 312 samples/day (HID36_POP4), ≥88 samples per day (standard sequencing with POP to 7™ and 50 cm capillaries) |
Capillary Length | 50 cm, 36 cm |
Format | 96-well plate, 8-well strips |
Number of Capillaries | 8 Capillaries |
Read Length | ≥420 bp using a 36 cm capillary and POP-4™, >850 bp using a 50 cm capillary and POP-7™ polymer |
Polymer | POP-4™, POP-6™, POP-7™ |
Polymer Consumption | Replace at earliest of 14 days⁄96 samples⁄12 injections (96-sample pouches), Replace at earliest of 14 days⁄384 samples⁄60 Injections (384-sample pouches), Replace at earliest of 14 days⁄960 samples⁄120 Injections (960-sample pouches) |
Warranty | 1 Year Limited Warranty on Parts and Labor, Service Installation and Application Training Included |
Dimensions (D x W x H) | Electrophoresis Unit Width (Closed Door): 61 cm, Electrophoresis Unit: 122 cm (W) (open door) x 61 cm (D) x 72 cm (H) |
Amperage | 15 A |
Voltage | Electrophoresis Voltage – Up to 20 kV |
External Computer | Hard Drive: 2x 500GB SATA 3.0 Gb/s and 8 MB Data Burst Cache, Memory: 16 GB (2x 8GB) 1600 MHz DDR3 Non-ECC. 4th Gen Intel Core I7 Processor- 3.1 GHz Turbo Processor, Operating System: Windows 7 SP1 |
Laser Features | Long-life single-line 505 nm solid-state laser excitation source., Long-life single-line 505 nm solid-state laser |
Operating Environment | Humidity: 20%-80% (non-condensing), Room temp.: <2° fluctuation during runs, Room temp: <2° fluctuation during runs, Temperature: 15°C-30°C, Humidity: 20–80% (non-condensing) |
Operating System | Windows 7 (Service Pack 1) |
Software | GeneMapper™ ID-X Software |
The 8-capillary 3500 Genetic Analyzer sets the standard in capillary electrophoresis. The 3500-series instruments are the first genetic analyzers designed with a specific feature set and workflow for the Human Identification application. The complete product combines the instrument with Applied Biosystems™ reagents, consumables and software, as well as industry leading support, to provide an integrated HID system solution that significantly improves ease-of-use and application efficiencies.
• Increased Consistency – Reduced signal variation with factory standardization and optional in-lane normalization
• Real-Time Data Quality Assessment – On instrument data evaluation features provide rapid identification and re-injection of samples requiring additional processing
• Improved Thermal Control – Smaller oven design and improved door seal for more consistent data migration
• HID Specific Workflow – Pre-configured for Applied Biosystems™ amplification kits to simplify run setup and software navigation
• Effortless Consumable Installation – Convenient one-time use consumable packaging
• Consumable Tracking with RFID Technology – On-instrument, real-time monitoring of consumable consumption and expiration
• Enhanced Throughput – Faster data generation with 8 or 24 capillary arrays and shorter run times
• Improved Polymer Pump Design – Reduced waste and fewer bubbles for better data quality
• Simplified Instrument Installation & Operation – New solid-state laser eliminates need for high voltage wiring and external venting
• Streamlined Instrument Maintenance – HID specific performance check and maintenance scheduling tools
• Data Chain of Custody – Controlled access and tracking through Security, Audit and eSig features
For Research Use Only. Not for use in diagnostics procedures.
The GlobalFiler Express PCR Amplification Kit and Prep-n-Go Buffer (for buccal swabs) combines the features of our GlobalFiler Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer (for buccal swabs). The GlobalFiler Express kit is the first 6-dye, 24-locus STR kit that enables direct PCR amplification, eliminating the need to perform DNA extraction or purification steps for DNA database and other single-source reference samples. Prep-n-Go Buffer (for buccal swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.
The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.
The Applied Biosystems HID NIMBUS Presto System is an easy-to-use, compact instrument that combines the KingFisher Presto Purification System with an industry leading automated liquid handling workstation to precisely purify nucleic acids from lysed samples for use in sensitive downstream analyses such as DNA quantification and STR amplification.
The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.
This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.
The Applied Biosystems SeqStudio Genetic Analyzer for HID is a low-throughput, easy-to-use, convenient benchtop system that delivers gold-standard fragment analysis or Sanger sequencing with just a simple click. It is validated across a broad range of HID forensic kits to deliver answers you can trust. The SeqStudio Genetic Analyzer couples the latest advancements in touchscreen usability with the same data quality, reliability, and support you have come to expect from Applied Biosystems instruments.
A36187
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A46208
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Amped Replay is an enhanced video player that allows investigators, frontline officers, first responders, security personnel and CCTV operators to conduct a quick first-level analysis of their videos, with easy conversion, quick corrections, redaction, and annotation functions, as well as a report, ideal for case building and disclosure. It includes Amped Software’s powerful DVR video conversion engine to view video without the need for other non-compatible, legacy, or proprietary player software. Its easy-to-use interface means no advanced skills are required to achieve professional results in the presentation of evidence, whether used as part of a case, forwarded to another unit or intelligence analysts, or sent to the media for identification. With Amped Replay, organizations are reassured that video is processed correctly at all levels, using validated forensic tools.
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