TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of soma in NGS – Next Generation Sequencing of Illumina
Illumina
Reagents
26 Variants detection from Somatic cells
TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of soma in NGS – Next Generation Sequencing of Illumina
TruSight Tumor 26 Kit include reagents to prepare for Library preparation samples, sequencing 26 genes relating to solid cancer, variant of soma in NGS – Next Generation Sequencing of Illumina
List | Parameter |
Time preparation | Total <7h (including, 2.5h to manual) |
Sequencing time | 22h in MiSeq system, upto 33h in HiSeq 2500 system |
Technology | Next Generation Sequencing |
Input sample | Based on QC, typically appx. 30-300 ng DNA |
Sequencing | 2 × 121bp |
15 gene được giải trình tự | AKT1, ALK, APC, NRAS, BRAF, CDH1, CTNNB1, EGFR,
ERBB2, FBXW7, FGFR2, FOXL2, GNAQ, GNAS, KIT, KRAS, MAP2K1, MET, MSH6, NRAS, PDGFRA, PIK3CA, PTEN, SMAD4, SRC, STK11, TP53 |
Amplicon | Include 174 amplicon with average size 165-195 bp |
Sample | FFPE |
Compatible NGS system | MiniSeq, MiSeq, MiSeqDx ở chế độ Reseach Mode, NextSeq 550 |
Sample running capacity | 4 sample/time on MiSeq48 sample running on HiSeq 2500 (rapid-run mode) |
Limit detection | 3% |
Frequency | ≤5% |
Coverage | Min 1000×, avg.7000× |
Variants detection | Indels, changing of genetics in Somatic cells |
Supplier
Illumina
The EntroGen NGS Targeted Hotspot Panel is a comprehensive assay that detects clinically relevant mutations in solid tumors using next-generation sequencing, compatible with fresh frozen and FFPE samples. It offers high sensitivity, low DNA input, and the ability to batch up to 12 samples in a single run, making it ideal for labs with limited sample volumes.
SOLIDaccuTest™ is an excellent tool to explorer variants associated with solid tumors using a comprehensive method of next-generation sequencing (NGS). It reflects the latest research trends and is optimized for the medical purpose by selecting essential genes of solid tumors including lung, colon, breast, skin brain, gastric, ovarian cancers, etc.
BRCAaccuTest™ & BRCAaccuTest™PLUS is a regent for producing libraries for
analyzing the BRCA ½ genes using the NGS (next-generation sequencing) method,
which analyzes genomic DNA derived from blood or FFPE tissues.
Model: NovaSeq 6000
Manufacturer: Illumina/USA
– Scalable platform
Match data output, time to results, and price per sample to study needs
– Flexible performance
Configure sequencing method, flow cell type, and read length to support a broad range of applications
– Streamlined operation
Increase lab efficiency with a simplified workflow and reduced hands-on time
The NextSeq 2000 Sequencing System uses patterned flow cells similar to those that power the NovaSeq™ 6000 System. The result is a highly flexible and scalable benchtop system that offers the highest cluster density flow cell of any on-market NGS system to date, driving down the cost per gigabase (Gb) of the sequencing run.
Model: NextSeq 1000
Manufacturer: Illumina
Origin: Singapore/United States
Launching year: 01/2020
PANA RealTyperTM HPV Kit is a CE marked diagnostic device in accordance with the European Union in vitro Diagnostic Medical Device Directive 98/79/EC.
DxFLEX is a new clinical flow cytometry platform derived from the successful CytoFLEX. The advanced sensitivity and intuitive software DxFLEX makes flow cytometry routine for both novice and expert flow cytometry technicians and promotes standardization. Functionality in the autoloader facilitates accurate results and sample tracking.
Model: CytoFLEX
Manufacturer: Beckman Coulter Life Sciences/ USA
The CytoFLEX Flow Cytometer is the most innovative flow cytometer system of Beckman Coulter. With compact design and high efficiency in work, CytoFlex provides and easy to use system with the impressive performance you need. Our system allows the researchers just focus on the science with no worry about instrument. Its superior sensitivity and resolution throughout all configurations give it the edge over other cytometry systems four times its size.
– Up to 3 lasers Violet-Blue-Red (V-B-R) and fully activated with 13 colors
– Ability to measure 100 nm particles with violet laser
– Includes 13 band pass filters: 450/45, 525/40 (2), 585/42, 610/20 (2), 660/10 (2), 690/50, 712/25, 780/60 (3)
– For higher throughput applications an optional plate loader module is available
– Smart software in acquisition and analysis CytExpert
TruSight Tumor 26 Kit includes library preparation reagent set and uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 26 genes that are commonly related to mutations in solid tumors and somatic variants