Zymo Research
Reagents
The Individual Fecal Collection Kit is a conveniently pouched kit that includes all the components needed for collecting and stabilizing fecal samples. This kit includes a Fecal Collection Tube, a Feces Catcher, a Biohazard Bag, Gloves, and multi-language instructions. Featuring DNA/RNA Shield technology, samples collected with the Fecal Collection Tube are accurately and safely preserved for downstream analysis.
| Device Specs | Individually pouched Kit that includes a Fecal Collection Tube (R1101), Feces Catcher (R1101-1-10), Biohazard Bag (P3000-1-10), Gloves, and Multi-language Instructions. |
|---|---|
| Sample Collection | 1g or 1mL stool sample |
| Sample Source | Stool |
| Sample Storage |
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| Stability | RNA: Ambient temperature (4°C- 25°C) at least 1 month DNA: Ambient temperature (4°C- 25°C) at least 2 years DNA & RNA: Frozen (< -20°C): Indefinitely |
| Cat # | Name | Size |
|---|---|---|
| R1180 | DNA/RNA Shield Fecal Collection Kit | 1 kit |
The ZymoBIOMICS DNA/RNA Miniprep Kit is designed for purifying DNA and RNA from a wide array of sample inputs (e.g. feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin Column technology that results in high-quality DNA and total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids). Ready for RT-PCR, arrays, sequencing, etc.
The ZymoBIOMICS Fecal Reference with TruMatrix™ Technology is a microbial reference material composed of stool from healthy donors for quality control, process validation, assay development, and proficiency testing. The fecal reference was homogenized in one large batch to produce consistency in every vial, eliminating typical lot-to-lot variability. With over 2 million preps available, this reference is intended to be used as a control for workflow performance, as well as comparing workflow consistency across replicates, studies, and laboratories. To facilitate methods comparison, a platform is in development to enable users to compare characterization data against applied methodology.
16S rRNA sequencing is a routine technique for microbiome composition profiling. Compared to shotgun metagenomics sequencing, 16S rRNA sequencing is more cost-effective and more robust; it generally requires less input DNA and is less impacted by the presence of host DNA. However, 16S rRNA sequencing has its own challenges. One major challenge is the formation of PCR chimeric sequences, which are artificial sequences resulting from the recombination of two or more PCR templates. Additionally, with common 16S primers, it is difficult to achieve both species-level resolution and broad phylogenetic coverage. Moreover, common 16S library preparation protocols used in the field have not been optimized to be cost-effective for large-scale applications. The Quick-16S NGS Library Prep Kit aims to standardize the library preparation process for 16S rRNA sequencing. Distinguishing features of the kit are described below. Fastest 16S rRNA Library Prep. The Quick-16S NGS Library Prep Kit utilizes real-time (quantitative) PCR (qPCR) rather than endpoint PCR for 16S rRNA amplification, enabling direct quantification of PCR products and eliminating the need for additional library quantification analysis such as TapeStation analysis or gel electrophoresis. An enzymatic clean-up is introduced between the two PCR steps, saving time and reducing costs as compared to lengthy AMPure bead-based clean-ups. With these features, the kit dramatically reduces the hands-on time of 16S library preparation. Simple. The Quick-16S NGS Library Prep Kit includes all the reagents needed to convert 96 DNA samples to a 16S library. The resulting library is directly compatible with the Illumina MiSeq without needing additional custom sequencing primers. Accurate. The utilization of real-time PCR also enables users to control PCR cycles. This limits chimera formation and PCR bias while obtaining enough products for subsequent sequencing. In most cases, the abundance of PCR chimeric sequences is maintained below 2%. Increased Coverage. Due to the rapid expansion of 16S rRNA databases, the insufficient microbial coverage of common 16S primer sets has become evident. Zymo Research has re-designed two common primer sets targeting the 16S V1-V2 and 16S V3-V4 regions based on the most updated 16S reference database and significantly improved their coverage.
